ERP Micro December 2019

end of analysis, as well as the results of a procedural control which is contained in every Amplification tube. Assurance® GDS for E. coli O157:H7 Tq method detects strains of E . coli O157:H7 and toxigenic E . coli O157:NM. This method is designed to be highly selective and does not detect microorganisms that are potential cross-reactors in antibody-based assays, including E . coli O157 that are not H7 or NM, and other microorganisms that express the O157 antigen but are not E . coli O157:H7. B. Apparatus Items are available from MilliporeSigma ( www.sigmaaldrich.com ) . Ordering information —SigmaAldrich, 12822 SE 32 nd St, Bellevue, WA 98005, Tel: 425-586-3300, email: bcs_cust_svc_bellevue@milliporesigma.com (a) Assurance® GDS Rotor-Gene® Q thermocycler – (Cat. No. 73070BC) (b) Laptop computer with Assurance GDS Rotor-Gene software – (Cat. No. 73071BC), version 2.3.103 (c) Assurance GDS Rotor-Gene Q Rotor-Disc – (Cat. No. 73039BC), 36-well (d) Assurance GDS Rotor-Gene Q locking ring (Cat. No. 73040BC), 36-well, to lock the Rotor-Disc (e) Adhesive film strips – (Cat. No. 73026BC), 200/case (f) Sample wells and sample wells base – samples wells (Cat. No. 73044BC), 200/case and sample wells base (Cat. No. 73043BC), 10/case (g) Resuspension plate – (Cat. No. 73006BC), 120/case (h) Gel cooling block – (Cat. No. 73083BC), box of 2 units (i) PickPen TM device – (Cat. No. 73091BC), 8 magnets (j) PickPen TM tips – (Cat. No. 73087BC), racked, 96/box (k) Adjustable micropipette – capable of accurately dispensing 1000 µL (l) 8-channel micropipette – capable of dispensing 30 µL (m) Filter barrier micropipette tips – (Cat. No. 73034BC), 10 – 50 µL, 960/case, or equivalent and (Cat. No. 73030BC), 200–1000 µL, 800/case, or equivalent (n) Adjustable repeat pipette – capable of dispensing 20 µL, 45 µL, 500 µL, and 1000 µL (o) Repeat pipette tips – (Cat. No. 73001BC), 0.5 mL, 100/case, or equivalent and (Cat. No. 73036BC), 10 mL, 100/case, or equivalent (p) Incubator – capable of maintaining and 42 ± 1 o C (q) Freezer – capable of maintaining -20 ± 2 o C

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