ERP Micro December 2019

(r) Refrigerator – capable of maintaining 5 ± 3 o C, for storing GDS kits and enriched samples (s) Microtiter plate vortex mixer – (Cat. No. 73063BC), IKA® MS 3 or equivalent, capable of maintaining 900 RPM (t) Laboratory paddle blender – Stomacher® 400 and 3500 or equivalent, for sample homogenization (u) Sterile laboratory filtered Stomacher bags – Nasco or equivalent (v) Top-loading balance – minimum 4,000 g capacity, sensitivity of 0.1 g (w) Digital countdown UP timer Optional: The following apparatus are required for use with Assurance® GDS for E . coli O157:H7 Tq assay (576 tests, Cat. No. 71007-576) due to the reduced size of the Amplification tubes. (b) Amplification tube holder lid – (Cat. No. 79184BC), variable spacing, 72-well (c) Amplification tube capping tool – (Cat. No. 79132BC) (d) Amplification tube cap rack – (Cat. No. 79197BC), 72-well (e) Aluminum cooling block – (Cat. No. 73093BC), 72-well (f) Assurance GDS Rotor-Gene® Q Rotor-Disc – (Cat. No. 73076BC), 72-well (g) Assurance GDS Rotor-Gene® Q locking ring (Cat. No. 73084BC), 72-well, to lock the Rotor-Disc (a) Amplification tube holder – (Cat. No. 79134BC), variable spacing, 72-well

Optional: Assurance® GDS PickPen TM PipetMax® (Cat. No. 79100BC)

For information on additional materials needed for sample analysis by the Assurance® GDS PickPen® PIPETMAX® (PPMX) please see the PPMX User Manual (No. 55240BC).

C. Media and Reagents Items are available from MilliporeSigma ( www.sigmaaldrich.com ) . Ordering information —SigmaAldrich, 12822 SE 32 nd St, Bellevue, WA 98005, Tel: 425-586-3300, email: bcs_cust_svc_bellevue@milliporesigma.com . (a) modified EHEC broth (mEHEC®) — Prewarm 1 L sterile deionized water at 42 ± 1˚C overnight. On day of use aseptically transfer 31.6 g mEHEC® media into the prewarmed sterile water. Gently mix to fully rehydrate the media. Use prepared broth within 6 h. Alternatively, mEHEC® broth can be prepared and autoclaved at 121˚C for 15 min. Autoclaved broth must be prewarmed to 42 ± 1˚C overnight prior to sample addition.

Page 10 of 34