ERP Micro December 2019

the PickPen TM tips against the side of the sample wells to remove excess media droplets. 8) Transfer the PickPen TM tips to corresponding sample wells containing Wash Solution and gently swirl for 5 – 10 sec. Tap the PickPen TM tips against the side of the wells to remove excess Wash Solution droplets. Note: Do not release particles into Wash Solution. 9) Transfer PickPen TM tips to the corresponding row of the prepared resuspension plate. With tips submerged, retract the PickPen TM magnets and tap tips gently to release particles into the Resuspension Buffer Tq. Cover the resuspension plate with adhesive film. 10) Repeat steps (6) through (9) for all samples using new tips for each strip of samples. Note: Amplification tube prep can also be completed using PPMX; for setup procedures please see the PPMX User Manual (No. 55240BC). Change gloves prior to handling reagents. 1) Prior to initial use, the gel cooling block must be stored in the freezer (-25 to -15°C) for at least 6 h. When frozen the gel cooling block will change color from pink to purple. When not in use the gel cooling block should continue to be stored at -25 to -15°C until it has turned completely purple, indicating it is ready to use. 2) The 72-well aluminum cooling block is for use with the 576 test kit and should be stored in the refrigerator (5 ± 3 °C). Between each use the aluminum cooling block should be returned to the refrigerator. 3) The Assurance® GDS Rotor-Gene® set up and data entry should be completed prior to transferring samples from the resuspension plate into the EHEC Amplification Tubes Tq. 4) Remove the Amplification Tubes from the foil pouch and place them in the frozen gel cooling block. Reseal and return the pouch to the refrigerator. 5) Transfer 30 µL of the sample from resuspension plate well into each Amplification Tube using a multi-channel pipette and filter barrier tips. Firmly press down on each Amplification Tube lid to close. Visually inspect each tube to ensure that the cap is securely sealed. 6) Place Amplification Tubes into the rotary ring in sequential order, beginning with position # and load into the Assurance® GDS Rotor-Gene®. 7) For the 576 kit, place amplification tubes into the aluminum cooling block. Place amplification tubes into Assurance ® GDS Rotor-Gene ® in sequential order, beginning with position #1. For the 100 test and ATM test kits, use the 36-well Rotor-Disc and locking ring; for the 576 test kit, use the 72-well Rotor-Disc and locking ring. (b) PCR Procedure

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