ERP Micro December 2019

MPN a / Test Portion or CFU b /Cloth

Candidate

Reference

Test Portion Size

Matrix

N c

dPOD CP g

95% CI h

x d POD CP e

95% CI

X POD CC f

95% CI

5 0

0.00 0.50 1.00 0.00 0.50 0.60 0.00 0.25 0.80

0.00, 0.43 0 0.30, 0.70 9 0.57, 1.00 5

0.00 0.45 1.00 0.00 0.50 0.60 0.00 0.25 0.80

0.00, 0.43 0.26, 0.66 0.57, 1.00 0.00, 0.43 0.30, 0.70 0.23, 0.88 0.00, 0.43 0.11, 0.47 0.38, 0.96

0.00 0.05 0.00 0.00 0.00 0.00 0.00 0.00 0.00

-0.47, 0.47 -0.16, 0.26 -0.47, 0.47 -0.47, 0.47 -0.13, 0.13 -0.47, 0.47 -0.47, 0.47 -0.26, 0.26 -0.45, 0.45

-

Frozen finely textured beef

20 10

375 g

0.00 (0.00, 0.09)

5 5

0.46 (0.20, 1.02)

5

0

0.00, 0.43

0

N/A i

Carcass cloth j

20 10

0.30, 0.70 10

1 cloth

0.80

5

3

0.23, 0.88

3

3.00

5 0

0.00, 0.43 0 0.11, 0.47 5 0.38, 0.96 4

-

Green onions

0.17 (0.03, 0.033) 20 5

25 g

5 4

2.00 (0.96, 4.13)

a MPN = Most Probable Number is calculated using the LCF MPN calculator provided by AOAC RI, with 95% confidence interval. b CFU/cloth = Results of the CFU/test area were determined by plating the inoculum for each matrix in triplicate. c N = Number of test portions. d x = Number of positive test portions. e POD C = Candidate method confirmed positive outcomes divided by the total number of trials. f POD R = Reference method confirmed positive outcomes divided by the total number of trials. g dPOD C = Difference between the confirmed candidate method result and reference method confirmed result POD values. h 95% CI = If the confidence interval of a dPOD does not contain zero, then the difference is statistically significant at the 5% level. I N/A = Not applicable. j MPN not available for carcass cloth. Low and high inoculation levels CFU/cloth indicated.

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