ERP_MicrobiologyMethods_Meeting_Book_11-2022

ERP Meeting Book - November 2022

( 10 ) Use the 7500 Fast 96W Strip Plate Adaptor to transfer the tubes to the instrument in the same configuration as the run layout. Use the Precision Plate Holder for SureTect™ assays. Be sure to load empty SureTect™ PCR tube strips as directed by the software. ( 11 ) Close the tray to the instrument and follow the RapidFinder™ Express Software prompts to start the run. Data analysis is automated by the software.

H. Test Result Report and Interpretation of Results

( a ) In the home screen of the RapidFinder™ Analysis Software (Figure 2021.06A ), click Results, then click the sub-tab for the desired view of the data.

( 1 ) Summary—plate format

( 2 ) Results—table format

( 3 ) Details—amplification plot

( b ) In the RapidFinder™ Express Software (Figure 2021.06B ), select View Results on the main page, select the appropriate run file, and follow the prompts to view results. To display a list of results in table format, click Table View. Select a sample, then click View Details to see replicate information about samples.

I. Candidate Confirmation

( a ) Streak 10 µL primary enrichment onto BLA (or equivalent) and incubating for 22–26 h at 36–38°C. ( b ) Confirm characteristic and well-isolated Listeria colonies using Microbact Listeria 12L Kit. ( c ) Characteristic colonies can also be confirmed using the methods described in, depending on the legislation territory or matrix:

( 1 ) FDA/BAM Chapter 10

( 2 ) EN ISO 11290-1

( 3 ) USDA/FSIS MLG 8.12 (8)

( 4 ) Any other appropriate national reference method

Or using:

( 5 ) Appropriate OMA-validated confirmation method

( 6 ) EN ISO 16140-6:2019 (9) validated confirmation method