ERP Proposal SPDS Set 2

Stakeholder Panel on Dietary Supplements Expert Review Panel for Set 2 Ingredients: Ashwagandha, Folin C and Kratom

PROPOSED CANDIDATES

Expected to Convene: December, 2015 AOAC INTERNATIONAL HEADQUARTERS Rockville, Maryland

AOAC INTERNATIONAL 2275 Research Blvd, Suite 300 Rockville, Maryland 20850 Phone: (301) 924-7077

MEMORANDUM

DATE :

November 5, 2015

TO:

Members of the Official Methods Board

FROM:

Christopher Dent, Standards Development Coordinator

SUBJECT: AOAC INTERNATIONAL AOAC Expert Review Panel for AOAC Stakeholder Panel on Dietary Supplements (SPDS) Set 2 Ingredients: Ashwagandha, Folin C, and Kratom BACKGROUND AOAC will convene the Expert Review Panel for AOAC SPDS Set 2 ingredients in December, 2015 at AOAC INTERNATIONAL Headquarters in Rockville, Maryland. The purpose of the meeting will be to review the methods submitted in response to the Call for Methods for Set 2 Ingredients. • ASH-01: Estimation of Withanolides (Withanoside IV, Withanoside V, Withaferin A, 12-Deoxywithastromonolide, Withanolide A, Withanolide B) in Withania somnifera o Submitted by Balasuramanian Murali, Natural Remedies, India • FOL-01: Single Reagent Folin o Submitted by Joe Vinson, University of Scranton, USA • FOL-02: METHOD FOR THE ESTIMATION OF TOTAL PHENOLIC CONTENT USING THE FOLIN- C ASSAY o Submitted by Jyotish Srivastava, OmniActive Health Technologies, India • FOL-03: Modified Folin-C Antioxidant Capacity Assay for Measuring Lipophilic Antioxidants o Submitted by Resat Apak, Istanbul University, Turkey • KRA-01: Quantitative and Qualitative Analysis of Mitragynine in ‘Kratom” (Mitragyna Speciosa) by GC-MS, LC-MS/MS and UPLC-PDA o Submitted by Christine Casey, US FDA • KRA-02: Quantification of Mitragynine in Kratom Raw Materials and Finished Products by High-Performance Liquid Chromatography: Single-Laboratory Validation o Submitted by Elizabeth Mudge, British Columbia Institute of Technology, Canada • KRA-03: Identification and Characterization of Indole and Oxindole Alkaloids from Leaves of Mitragyna speciosa Korth Using Liquid Chromatography o Submitted by Iklas Khan, University of Mississippi, USA • KRA-04: LC/MS Method for the Identification of Mitragyna speciose (Kratom) and Quantitiation of Mitragynine Using Linear Ion Trap Mass Spectrometer o Submitted by Teresa Cain, US FDA LIST OF METHODS:

RECOMMENDATION

The following twenty one (21) candidates are being submitted for consideration by the Official Methods Board to evaluate candidate methods for Ashwagandha, Folin-C and/or Kratom. We are currently proposing Darryl Sullivan as the Chair for this entire Expert Review Panel. Many of the following candidates have participated in various AOAC activities, including but limited to, prior Expert Review panels and AOAC standards development activities. All proposed candidates will be required to attend the mandatory AOAC Expert Review Panel Orientation webinar that will be held during the month of November, 2015. Incoming Expert Review Panel Chairs will also be trained on the AOAC Expert Review Panel process. ASHWAGANDHA FOLIN-C KRATOM

Anton Bzhelyansky

USP

Nour Eddine Es-Safi

Mohammed V University in Rabat

Christine Casey

FDA

MURALI BALASURAMANIAN

NATURAL REMEDIES

John Finley

Louisiana State University

Nour Eddine ES-SAFI

Mohammed V University in Rabat

Premal Bhatt

USP

Prashant Ingle Martha Jennens

Herbalife Covance

Charles Metcalfe

Custom Analytics

Nour Eddine ES-SAFI

Mohammed V University in Rabat

Tom Phillips

State of Maryland

Prashant Ingle

Herbalife

Dana Krueger

Krueger Food Laboratories

Catherine Rimmer

NIST

Tom Phillips

State of Maryland Jungmin Lee

USDA

Darryl Sullivan

Covance

Catherine Rimmer

NIST

Tom Phillips

State of Maryland

John Szpykla

Merieux Nutrisciences

Casey Sayre

Roseman University of Health Sciences

Catherine Rimmer

NIST

Yanhong Wang

University of Mississippi

Aniko Solyom Darryl Sullivan

GAAS Analytical

Aniko Solyom Darryl Sullivan

GAAS Analytical

Covance

Covance

Kurt Young

GNC/Nutra Manufacturing

Joseph Zhou

Sunshineville Health

Yanjun Zhang

Herbalife

ALL APPLICANTS

Last Name

First Name Organization

Region

NATURAL REMEDIES

Industry

India

BALASURAMANIAN MURALI

USA USA USA USA USA USA USA USA USA USA USA USA USA USA USA USA USA USA USA

USP USP FDA

NGO NGO

Bhatt

Premal Anton

Bzhelyansky

Casey

Christine

Government

ES-SAFI

Nour Eddine Mohammed V University in Rabat Academia

Morocco

LSU / Retired

Academia

Finley

john

Ingle

Prashant

Herbalife Covance

Industry Industry Industry

Jennens Krueger

Martha

Krueger Food Laboratories

Dana

Lee

Jungmin charles

USDA

Government

Industry

metcalfe phillips Rimmer

custom analytics

scs mda

Government Government

tom

NIST

Catherine

Sayre

Casey Aniko

Academia

Roseman University of Health S i GAAS Analytical

Industry Industry Industry

Solyom

Sullivan Szpylka

Darryl

Covance

John

Merieux Nutrasciences University of Mississippi GNC/Nutra Manufacturing Herbalife International

Wang Young Zhang

Yanhong

Academia

Kurt

Industry Industry

Yanjun Joseph

Sunshineville Health Products, Inc Industry

Zhou

Major Perspectives

Academia Government Industry NGO

Specific Perspectives

Academia - Domestic

Academia - International

Government - Federal

Government - State

Industry - CRO

Industry - Food Company

Industry - Supplement Company

ASHWAGANDHA

Anton Bzhelyansky USP MURALI BALASURAMANIAN NATURAL REMEDIES Premal Bhatt USP Nour Eddine ES‐SAFI

Mohammed V University in Rabat

Prashant Ingle Tom Phillips

Herbalife

State of Maryland

Catherine Rimmer

NIST

Casey Sayre Aniko Solyom Darryl Sullivan

Roseman University of Health Sciences

GAAS Analytical

Covance

Kurt Young

GNC/Nutra Manufacturing

Yanjun Zhang

Herbalife 

Ashwagandha ‐ By Broad Perspectives ACADEMIA GOVERNMENT INDUSTRY NGO

17%

16%

Ashwagandha ‐ By Region USA INDIA MOROCCO

17%

8%

50%

9%

Ashwagandha ‐ By Specific Perspectives CRO MANUFACTURER REFERENCE MATERIALS REGULATOR RESEARCH

83%

17%

17%

8%

33%

25%

Nour Eddine Es‐Safi f\FOLIN-C

Mohammed V University in  Rabat Louisiana State University

John Finley

Prashant Ingle Martha Jennens Dana Krueger

Herbalife Covance

Krueger Food Laboratories

Jungmin Lee Tom Phillips

USDA

State of Maryland

Catherine Rimmer

NIST

Aniko Solyom Darryl Sullivan Joseph Zhou

GAAS Analytical

Covance

Sunshineville Health

Folin C ‐ By Broad Perspectives ACADEMIA GOVERNMENT INDUSTRY

Folin C ‐ By Specific Perspectives

CRO

IMPORTER

MANUFACTURER

REFERENCE MATERIALS REGULATOR

RESEARCH

18%

18%

55%

27%

37%

18%

9%

9%

9%

Folin C ‐ By Region

MOROCCO USA

9%

91%

KRATOM

Christine Casey

FDA

Nour Eddine ES‐SAFI

Mohammed V University in Rabat

Charles Metcalfe

Custom Analytics State of Maryland

Tom Phillips

Catherine Rimmer

NIST

Darryl Sullivan John Szpykla Yanhong Wang

Covance

Merieux Nutrisciences University of Mississippi

Kratom B Broad Perspecti es  ‐ y  v

Academia Government

Industry

Kratom ‐ By Specific Perspectives CRO Reference Materials Regulator Research

25%

38%

13%

37%

37%

38%

12%

Kratom ‐ By Region

Morocco USA

12%

88%

AOAC Expert Review Panel for AOAC Stakeholder Panel on Dietary Supplements (SPDS) Set 2 Ingredients Summaries of Expertise

BALASURAMANIAN, MURALI Area of Expertise: Quality assessment of Indian Botanicals

I have total experience of over 29 years in the field of Testing Similar experience in chromatograpic techniques. I have been associated with Natural Remedies (Herbal product manufacturing organisation) for about 19 years, where we test the quality of botanicals extracts and their formulations. In the past 19 years my laboratory personnel have tested over 1500 batches Ashwagandha roots and its extracts. We are the leaders in commercially introduce chromatographic methods for evaluation of quality. Developed and validated over 50 chromatographic methods including that of Ashwagandha. Submitted about 100 monographs to IP (Indian Pharmacopoeia) Ayurvedic Pharmacopoeia of India and USP. Analytical method validation for Assay and identity of most of the monographs of Indian medicinal plants (about 12 plants approximately 40 monographs) in USP is provided by us. We have expertise in testing Identity ,Assay, Heavy metals solvent residue Mycotoxins and Pesticides residues. Natural Remedies also supply the Phytochemical reference substance of many Indian botanicals including Ashwagandha. BHATT, PREMAL Area of Expertise: Varied Premal is an Associate Scientific Liaison for the Food Program Unit at US Pharmacopeial Convention (USP) since 2011. Previously, he worked as a Scientist II for USP’s R&D Laboratory from 2005 to 2011. As a Scientific Liaison-Foods, Premal is engaged in developing standards for identity, purity, and authenticity of food components. One of the areas of Premal’s interest is developing such standards for various spices. Premal has a background in analytical chemistry with more than 25 years of experience in various laboratory settings, such as pharmaceutical, chemical, and waste management. Premal holds two Master of Science degrees in Plant Pathology; one from Kansas State University and one from Gujarat Agricultural University, Anand, India. Premal has authored ten research papers in peer-reviewed journals. My laboratory background is in method development: I have been engaged in chromatographic and spectroscopic method development for about 12 years, of which roughly 3 years in generic pharma (LC, GC, titrimetry, IR) and the rest – working for a dietary supplement manufacturer (LC, UPLC, IC, GC, GC-MS, SEC, ICP-OES, NIR, IR, KF, ISE, dissolution). I also spent about 13 years at NIH performing separations of biologically-derived compounds, extracting analytes of interest from physiological fluids, setting up and optimizing bioassays such as ELISA. The largest segment of my time was spent on analysis of dietary supplement ingredients and finished products, both of botanical and non- botanical origin. I have worked to develop the current iteration of chondroitin CPC titration (as published in USP), developed and validated methods for MSM and oil-soluble vitamins, developed and optimized a fast Omega-3 marine oils analysis (poster at AOAC 2011), re-optimized peroxide, acid and anisidine value analyses; I was the AOCS approved chemist in AOCS/GOED Nutraceutical Oils in 2011. I also established in-house metals analysis by ICP-OES, and built incoming raw materials NIR library. Of the botanical analytes, I have hands-on experience with milk thistle, curcuminoids/turmeric, green tea extract, cranberry extract and a suite of methods for cruciferous analytes. The above list is not exhaustive. FULL CV: http://www.jotform.com/uploads/christopherdent/51735966869173/320777138031109893/resume (1).pdf FULL CV: http://www.jotform.com/uploads/christopherdent/51735966869173/320733235947214614/P. Bhatt.Resume.pdf BZHELYANSKY, ANTON Area of Expertise: Methodologies in Dietary Supplements

I have provided analytical support to finished dosage formulators, and actively participated in formulation,

performance evaluation and manufacture of the novel enterically-coated dosage form. I have not worked hands-on with W. somnifera during my time at the bench, however, the USP Ashwagandha monographs (Ashwagandha Root, Powdered Ashwagandha Root and Powdered Ashwagandha Root Extract) are my current responsibility; I have been collaborating with sponsors on monograph revisions, and reviewed significant volume of literature. An updated HPTLC identification method [Pharmacopeial Forum 41(5)], a soon-to-be-published new HPLC limit test, as well as the two concomitant Ashwagandha reference standard materials to be issued in the nearest future, have resulted from my engagement with the sponsors.

I hope that the Chief Science Officer and the AOAC OMB will find this information relevant in evaluating my candidacy for the Ashwagandha ERP.

FULL CV: http://www.jotform.com/uploads/christopherdent/51735966869173/320842827947794266/Anton Bzhelyansky - CV 2015 (October).docx

CASEY, CHRISTINE Area of Expertise: Dietary Supplements and Instrumentation

Ms. Casey expertise is with dietary supplements (botanical products) and the analytical instrumentation to determine the active compounds of interest. Ms. Casey worked at a startup company located at the University of Colorado Health Sciences BioScience Complex and developed methods for the quantification of natural bioactive polyphenol compounds in Traditional Chinese Medicine (TCM) by LC-MS/MS. Ms. Casey’s continued these are of expertise and work on the method development/validation for the Determination of Kratom in Herbal Products, and Identification of 5-Hydroxytryptophan in Herbal Supplements.

FULL CV: http://www.jotform.com/uploads/christopherdent/51735966869173/320749972841507360/CRC CURRICULLUM VITAE FY15.doc

ES SAFI, NOUR EDDINE Area of Expertise: Technologies for Analyzing Ingredients

In connection with the question about my expertise appropriate to the ERP under formation, I am hereby stating that my expertise is with the technologies and the methods used for the detection, the qualitative and the quantitative analysis of the ingredients.

FULL CV: http://www.jotform.com/uploads/christopherdent/51735966869173/320644846801459020/CV_EsSafi.pdf

FINLEY, JOHN Area of Expertise: Antioxidant Methods, Folin C

Historically I have organized two international symposia on antioxidant methods. I also have spoken several times at ACS and IFT concerning antioxidant methods. Perhaps most important on the Folin-C issue, I led the development of the SPDS Folin-C Working Group and request for methods proposal for AOAC.

FULL CV: http://www.jotform.com/uploads/christopherdent/51735966869173/318047971181188068/Finley Biosketch 2014 .doc

INGLE, PRASHANT Area of Expertise: Ashwagandha and Folin C Methodologies Ashwagandha: Worked with withanolides in Withania genus plants, experienced in evaluating LC-MS, HPTLC methods, advanced analytical techniques ELISA and sequencing based assays Folin C: Experience in performing and managing Antioxidant capacity and phenolic assays by ORAC, Trolox and HAT reaction, Chemiluminescence methods. Standardization of Folin Lowry method for various GAE in various antioxidant compounds and products FULL CV: http://www.jotform.com/uploads/christopherdent/51735966869173/320751646828513678/Prashant Ingle Resume AOAC ERP.doc

JENNENS, MARTHA Area of Expertise: Dietary Supplements and Testing

My experience with Folin C has been in testing nutritional supplements and finished products. The group I was in prior to technical development here at Covance was the group responsible for testing polyphenols using that method/reagent. I also served as the technical leader for that group and dealt with various client requests and

questions around the testing. Recently, during the working group meetings, I did some additional work for the working group in order to help decide the scope of the SMPR with various standard materials.

FULL CV: http://www.jotform.com/uploads/christopherdent/51735966869173/320642371890881076/MJC.doc

KRUEGER, DANA Area of Expertise: Folin-C My lab has used the Folin-Ciocalteu phenolics method for more than 20 years, particularly with reference to phenolics analysis of beverages

FULL CV: http://www.jotform.com/uploads/christopherdent/51735966869173/322580474781330265/Kreuger_CV.doc

LEE, JUNGMIN Area of Expertise: Total Phenolics/FolinC I have working knowledge of Folin-Ciocalteu (also known as total phenolics; AOAC abbreviated as Folin-C) method and published numerous papers using this method as indicated in my accompanying CV.

FULL CV: http://www.jotform.com/uploads/christopherdent/51735966869173/317033096981440983/Jungmin Lee AOAC FC CV.pdf

METCALFE, CHARLES Area of Expertise: Kratom

We have been analyzing Kratom raw materials and finished products for over 2 years. Initially we ran samples by conventional HPLC with a run time of 30 minutes. We found this run time to be unacceptable due to the number of samples we were required to assay. Our new methodology utilized UHPLC with a reduced run time of less than 8 minutes. All of the relevant alkaloids are separated. Althought the 7-OH mitragynine is of the most interest to regulatory agencies, we have yet to find any of this isomer in either raw materials or finished products. A sample chromatogram of our new assay is attached.

FULL CV: http://www.jotform.com/uploads/christopherdent/51735966869173/319343104296175371/CEM Resume.doc

PHILLIPS, TOM Area of Expertise: Various Methodologies

I have over 20 years Mass Spectrometry experience; ten of which are with hyphenated techniques, i.e., ms/ms, bot GC and LC. I have over 4 years of experience with herbal products, both the raw material and the finished products. I have developed in-house method for the determination of actives in herbals, but I do not have experience with these particular ones. I have done Folin C in several matrices for about 5 years or so.

FULL CV: http://www.jotform.com/uploads/christopherdent/51735966869173/320642535762514400/resumechemist.doc

RIMMER, CATHERINE Area of Expertise: Various Methodologies

My experience is with the methodologies (especially chromatography, absorption detection, fluorescence detection and mass spectrometric detection) and the statistics utilized for quantitation and qualitative identity testing. As the dietary supplement program coordinator at NIST, I keep up on dietary supplement matricies, analytes, and methods.

FULL CV: http://www.jotform.com/uploads/christopherdent/51735966869173/322884857781732601/Rimmer CV (2).pdf

SAYRE, CASEY Area of Expertise: instrumentation and botanicals; method development & validation

I have experience with some of the instruments and techniques that could be used to detect or determine the ingredients in the call for experts. These include HPLC, mass spectrometry, and UV spectrophotometry. Additionally, I have experience with botanical sample extraction and preparation for quantitative analysis. I also have experience in the development and validation of stereospecific quantitative methods of detection of plant secondary metabolites in aglycone and glycoside forms. Most of the analytes I have experience with are chiral polyphenols.

FULL CV: http://www.jotform.com/uploads/christopherdent/51735966869173/317033339622225640/CV_Sayre_1SEP2015.pdf

SOLYOM, ANIKO

I have more than 30 years of comprehensive experience in analytical method development and validation, using a wide variety of analytical techniques. My expertise is in development and validation of analytical methods with particular emphasis on HPLC, LC-MS applications and bioactivity guided preparative separation of complex mixtures and analysis of biological samples and dietary supplements. In addition to HPLC technique I have significant experience with atomic absorption spectroscopy (AA), inductively coupled plasma emission spectroscopy (ICP), photoacoustic spectroscopy (PA), UV-Vis spectroscopy, elemental analysis, mass spectrometry (MS), gas chromatography (GC), GC-MS, LC-MS, NMR and wet techniques. Before founding GAAS Corporation/GAAS Analytical, I served as director of the Analytical Core at an NIH funded Botanical Center (Arizona Center for Phytomedicine Research). The Facility focused on quantitation and identification of natural products and dietary supplements, particularly anti-inflammatory compounds from turmeric, ginger and boswellia. I developed methods to analyze these compounds from the plant materials, extracts, commercially available dietary supplements and from different biological matrices. I also developed and validated new assay methods according to cGLP regulations, to quantify novel cancer drugs in plasma and urine using QQQ LC/MS technique; identifying metabolites of new cancer drugs in plasma and urine using TOF LC/MS technique and calculating pharmacokinetic parameters. I am an active member of AOAC International; I joined the organization in 2002. Currently I am member of AOAC’s Stakeholder Panel on Dietary Supplements and member of various working groups. I have participated in multiple AOAC organized collaborative studies (glucosamine, ginkgo, saw palmetto) in order to validate analytical methods to be used by the dietary supplement industry. I was Horwitz advisor of AOAC until the restructuring the Official Methods of Analysis (OMA) process in January 2008, when the role of method advisors and Horwitz advisors was retired. For 5 years I have been serving as member of the Presidential Task Force on Dietary Supplements of AOAC, and also served as member of Expert Review Panels of AOAC. I was selected in June 2015 to serve a 5 year term as the member of USP’s Non-botanical Dietary Supplements Expert Committee. FULL CV: http://www.jotform.com/uploads/christopherdent/51735966869173/319015450438307049/Curriculum_vitae_Aniko Solyom_ March 2015.pdf Darryl Sullivan is a Fellow of AOAC and has been an active member since 1980. He has served terms as secretary, president-elect, president, past president, and director of the Board of Directors, and previously served a three-year term as chair of the Official Methods Board, and is currently serving as Chair of the AOAC Stakeholder Panel on Infant Formula and Adult Nutritionals. In 2012 Darryl lead a very successful AOAC engagement with government and industry thought leaders in India and China on behalf of SPIFAN. He is also active with the Stakeholder Panel for Strategic Food Analytical Methods and the Stakeholder Panel for Agent Detection Assays. Sullivan also served a three-year term as a director on the AOAC Research Institute Board of Directors. He was a founding member and chair of the Presidential Task Force on Dietary Supplements and a member of the Task Force on Bacillus anthracis, as well as the AOAC Task Force on Nutrition Labeling and the AOAC Task Force on Sulfites. Prior to chairing the OMB, he served as a member and chair of the Methods Committee on Commodity Foods and Commodity Products. Sullivan was a founding member of the AOAC Technical Division on Reference Materials and served three terms on the Division's Executive Board. A staunch supporter of the Association, Sullivan was active in the e-CAM and Scholar I projects at AOAC, has exhibited at the annual meetings for many years, has presented hundreds of papers and posters at AOAC meetings and regularly publishes his research in the journal of the AOAC. He has also presented a significant number of papers on behalf of AOAC at other scientific meetings in many different parts of the world. SULLIVAN, DARRYL

FULL CV: http://www.jotform.com/uploads/christopherdent/51735966869173/322906973781589371/Sullivan, Darryl CV.pdf

SZPYKLA, JOHN

FULL CV: http://www.jotform.com/submissions/51735966869173

WANG, YAN-HONG

As a senior research scientist at the National Center for Natural Products Research, Dr. Yan-Hong Wang has been doing research on analytical/bio-analytical studies of medicinal plants and related products (dietary supplements). He has extensive experience in analytical chemistry, especially in liquid chromatography (LC) and mass spectrometry (MS), and strong background in methods development and validation using HPLC/UHPLC coupled with different detectors, such as PDA, MS and ELSD. Additionally, Dr. Wang has more than 15 years hand-on experience in natural products chemistry for isolation, purification and structural elucidation of active constituents from different natural sources using column chromatography, NMR, LC-MS/MS, UV-VIS and IR techniques.

FULL CV: www.jotform.com/uploads/christopherdent/51735966869173/322921711012537572/Yan- Hong%20Wang%20CV%2009November2015.pdf

YOUNG, KURT Area of Expertise: Ashwagandha method development and analysis For greater than 15 years at a major supplement manufacturing facility I have:

Overseen method development, quality specification confirmation and identity testing with Ashwaganda (Withania somnifera) extract and powdered herb incoming raw materials and finished products. Interacted with major suppliers of standardized extracts and powdered herb material to determine best methodology to support product development and quality control testing.

FULL CV: http://www.jotform.com/uploads/christopherdent/51735966869173/320148913132890861/KYoung_CV.docx

ZHANG, YANJUN Area of Expertise: methodologies and dietary supplements

Yanjun Zhang, has been trained in a Pharmacy School 30 years ago (1985) and has been working in the field of botanical natural product analysis since then, including natural products isolation, structure determination, quantitative and qualitative analysis of natural products, new drug development from Herbal medicine, etc. I have been using and guiding to use numerous analytical instruments and techniques, such as HPLC, LC-MS, GC, GC MS, NMR, NIR, and HPTLC etc. The natural products I’ve been studied include flavonoids, alkaloids, terpenoids, lignans, saponions, essential oils, sugars, organic acids, fatty acids, and polysacchrides etc. Currently I guide establish the Qualitative and Quantitative analytical method in Herbalife Quality group for internal use. With all the expertise mentioned above, I believe I can make great contribution to the review panel, and most important I want to contribute my expertise on the biological botanical natural products analysis to the AOAC Expert Review Panel for Dietary Supplement Ingredients.

FULL CV: http://www.jotform.com/uploads/christopherdent/51735966869173/320666720739751921/YANJUN ZHANG CV 2014.pdf

ZHOU, JOSEPH Area of Expertise: dietary supplements and method development

I am currently the technical director of Sunshineville Health Products, Inc, a dietary supplement manufacturer in state of West Virginia. I have been working in the US dietary supplement industry since 1995, and was a technical director of NOW Foods and Nature's Sunshine Products, respectively, in charge of dietary supplement analytical methods development, GMP and quality control. I have been actively participating in the AOAC dietary supplement official method program since 2000. I was the AOAC study director and SLV coordinator of the glucosamine method, and one of the SLV coordinators for the chondroitin method, as well as an ERP member for many AOAC methods. I received a special recognition: AOAC 2005 study director of the year.

I am willing to continue to make my contribution to the AOAC official method program for dietary supplements, and believe I have the technical capability to be an ERP member for many dietary supplement analytical methods.

FULL CV: http://www.jotform.com/uploads/christopherdent/51735966869173/320648427679132288/Joseph Zhou CV.pdf