EtOH in Kombucha SMPR V4
DRAFT AOAC SMPR 2016.XXX; Version 4; December 9, 2015. 1 2 Standard Method Performance Requirements for Determination of Ethanol in Kombucha 3 4 5 Intended Use : Use by trained technicians in a laboratory for routine quality assurance testing.
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1. Applicability :
Determination of low levels of ethanol as expressed as alcohol by volume (ABV) in
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2. Analytical Technique :
Any analytical technique that meets the following method performance requirements is
3. Definitions :
Alcohol by volume (%ABV)
A standard measure of how much alcohol (ethanol) is contained in a given volume of an
alcoholic beverage (expressed as a volume percent).
The 2-carbon alcohol with a molecular formula of CH 3 CH 2
OH. CAS Registry Number: 64-17-5
Kombucha is a fermented, effervescent tea beverage made by adding a symbiotic culture of bacteria and yeast (SCOBY) to a solution of tea and sugar, and may include other
Limit of Quantitation (LOQ)
The minimum concentration which quantitative results may be obtained with 95%
Variation arising when all efforts are made to keep conditions constant by using the same instrument and operator and repeating during a short time period. Expressed as the
repeatability standard deviation (SD r
); or % repeatability relative standard deviation
The standard deviation or relative standard deviation calculated from among-laboratory
data. Expressed as the reproducibility standard deviation (SD R
); or % reproducibility relative
standard deviation (% RSD R ).
The fraction or percentage of the analyte that is recovered when the test sample is analyzed
using the entire method.
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