Expert Review Panel for Dietary Supplements
AOAC DECEMBER 2017 ERPs - METHOD REVIEW FORM
Submission Date
2017-12-12 13:01:17
AOAC Expert Review Panel: Method Review
Reviewer Information Required to validate your review.
Name
SNEH BHANDARI
sneh.bhandari@mxns.com
Organization
Merieux NutriSciences
Remember, the main purpose of your review is to ensure the method conforms to the applicable SMPR. View and download SMPRs here:
SPDS SMPRs SPSFAM SMPRs
Method Review
Title of Method
Identification of Pea, Rice and Soy Proteins in Raw Materials and ------------------ --HPLC-MS/MS
AOAC Candidate Method Number (e.g. ALN-01)
PRO-001
Applicable SMPR
AOAC SMPR 2016.016
I. Summary of the Method
I. Summary of the Method
The samplle is extracted in 5 mM Urea Tris buffer followed by reduction with DTT and alkylation with iodoacetamide. The extract is digested with trypsin at 37C overnight abd quenched by formic acid.The extract is filtered and diluted with 0.1% formic acid and internal std (Beta-Casomorphin 1-4 peptide) and analyzed by LC-MS/MS. Pea, rice and soy protein identifications were based on the comparison with MRL sample, which is made from raw materials identified using PCR.
II. Review of the Method Only:
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