Expert Review Panel for Dietary Supplements
AOAC DECEMBER 2017 ERPs - METHOD REVIEW FORM
Submission Date
2017-12-13 15:36:52
AOAC Expert Review Panel: Method Review
Reviewer Information Required to validate your review.
Name
Philip Haselberger
philip.haselberger@abbott.com
Organization
Abbott Nutrition
Remember, the main purpose of your review is to ensure the method conforms to the applicable SMPR. View and download SMPRs here:
SPDS SMPRs SPSFAM SMPRs
Method Review
Title of Method
Identification of Milk Proteins in Raw Materials and Finished Goods using ESI HPLC-MS/MS
AOAC Candidate Method Number (e.g. ALN-01)
PRO-002
Applicable SMPR
2016.015
I. Summary of the Method
I. Summary of the Method
Method is based on an UPLC-MS/MS analysis of peptides after the sample has been through a trypsin digestion. Positive ID of the presence of milk protein at >0.1% is made by comparison relative to a lab prepared MRL sample that is nominally 0.1%. The presence of milk protein is only confirmed if 3 signature peptides from alpha-S1-casein and beta-lactoglobulin are all present. Otherwise the protein is not confirmed.
II. Review of the Method Only:
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