Expert Review Panel for Kombucha Tea

AOAC SPDS ERP REVIEW: MAIN FORM

Submission Date

2017-09-18 09:24:21

Name

Armen Mirzoian

E-mail

armen.mirzoian@ttb.gov

Organization

TTB

Title of Method

Determination of Ethanol in Kombucha by GC-FID

AOAC Candidate Method Number (e.g. ALN-01)

KOM-4

Applicable SMPR

2016.001

Summary:

The method measures concentration of ethanol in Kombucha by liquid injection GC- FID using 2-propanol as internal standard

1. Does the applicability of the method support the applicability of the SMPR? If not, please explain what is missing. 2. Does the analytical technique(s) used in the method meet the SMPR? If not, please specify how it differs from what is stated in the SMPR. 3. Are the definitions specified in the SMPR used and applied appropriately in the method? If no, please indicate how the terms are used. 4. Does the method, as written, contain all appropriate precautions and warnings related to the method's reagents, components, instrumentation, or method steps that may be hazardous? If no, please suggest wording or option(s). 1. Are the definitions specified in the SMPR used and applied appropriately in the supporting documentation (manuscripts, method studies, etc...)? If not, please explain the differences and if the method is impacted by the difference.

Generally it does, however it's not clear how are the LOQ and LOD evaluated.

No 1. Since determination of LOQ and LOD is not described, it is not clear whether they meet SMPR. 2. Recoveries for ethanol concentrations below 0.5% abv were not determined 3. Repeatability of the method (presented as Precision) exceeds 4% 4. Reproducibility was not determined

1. LOQ and LOD are neither defined nor evaluated. 2. Spike recovery determination is incomplete 3. Precision is only expressed as repeatability 4. Reproducibility was not determined

yes

Same as II.3. 1. LOQ and LOD are neither defined nor evaluated. 2. Spike recovery determination is incomplete 3. Precision is only expressed as repeatability 4. Reproducibility was not determined