Expert Review Panel for Kombucha Tea

5. Based on the supporting information, what are the pros/strengths of the method? 6. Based on the supporting information, what are the cons/weaknesses of the method?

GC-FID is widely available in many analytical laboratories; although the sample preparation step is not described, most likely it's dilute and shoot.

Potentially long analyses time (GC program is not described); small kombucha producers may not have access to GC-FID

7. Any general comments about the method?

In addition to the comments above:

1. The text states only two calibration standards at 0.1% and 0.5% abv. 2. Simple calculations of the calibration standard preparation procedure gives 5% and 25% abv solutions, however 0.1% and 0.5% abv is stated 3. It is not clear how calibration standards in the measured 0.1%-2% abv range were prepared. 4. Specificity and robust of the method was not addressed?

Do you recommend this method be adopted as a First Action and published in the Official Methods of Analysis of AOAC INTERNATIONAL? Please specify rationale.

No