Expert Review Panel for Kombucha Tea

AOAC SPDS ERP REVIEW: MAIN FORM

Submission Date

2017-09-12 16:28:27

Name

Sneh BHANDARI

E-mail

SNEH.BHANDARI@MXNS.COM

Organization

Merieux NutriSciences

Title of Method

Determination of ethanol in Kombucha by Gas Chromatography-Flame Ionization Detector: Intra-Laboratory Validation

AOAC Candidate Method Number (e.g. ALN-01)

KOM-04

Applicable SMPR

AOAC SMPR 2016.001

Summary:

Low level of ethanol is detected in Kombucha by gas chromatography with flame ionization detection employing 2-propanol as an internal std. Applicable for determination of 0.1-2% ABV ethanol in Kombucha tea beverage. LOD and LOQ = 0.003 and 0.011 %ABV respectively. The method precision as RSDr was found to be 0.55-2.5% and % spike recovery in the range of 99.-99.5% at different spike levels.

1. Does the applicability of the method support the applicability of the SMPR? If not, please explain what is missing. 2. Does the analytical technique(s) used in the method meet the SMPR? If not, please specify how it differs from what is stated in the SMPR. 3. Are the definitions specified in the SMPR used and applied appropriately in the method? If no, please indicate how the terms are used. 4. Does the method, as written, contain all appropriate precautions and warnings related to the method's reagents, components, instrumentation, or method steps that may be hazardous? If no, please suggest wording or option(s).

Yes

Yes

Yes

No. The precautions in use of flammable solvents in the method may be stated. The precautions and warning in use of gases under pressure may also be mentioned.