Expert Review Panel for Kombucha Tea

3. Is there information demonstrating that the method system suitability tests and controls as specified in the SMPR worked appropriately and as expected? If no, please specify. 4. Based on the supporting information, is the method written clearly and concisely? If no, please specify the needed revisions. 5. Based on the supporting information, what are the pros/strengths of the method? 6. Based on the supporting information, what are the cons/weaknesses of the method?

The method does not indicate any system suitability criteria.

The method as written seems not be complete. It does not provide details about how the samples are prepared for analysis. The details of the amount addition of internal std. in the sample and calibrartion std. missing.

The supporting data don't provide complete details about the method, i.e., analytical range of the method, what samples were used for the spike recovery evaluation etc.

Simple and easy method to operate. Spike recovery satisfactory (but sample details missing).

1. No data available to know the method precision in analysis of ethanol in Kombucha Tea. 2. The method provide no information to rule out interference from the components of Kombucha tea in accurate and precise quantitation of ethanol in samples. 3. The method accuracy not established using Certified ref materials. 4.Information not available to rule out the possibility of carry over from injection to injection. 5.Retention time of internal std and ethanol are close. The available data on the method performance don't rule out that this does not impact the accuracy of the method particularly in ethanol estimation at the low levels. 6. Information about the Analytical Range of the method missing.

7. Any general comments about the method?

The method performance evaluation is not yet complete. The method as written is not complete and missing many of the critical

Do you recommend this method be adopted as a First Action and published in the Official Methods of Analysis of AOAC INTERNATIONAL? Please specify rationale.

Not without getting additional details about the missing information in the method and its performance evaluation.