Expert Review Panel for Kombucha Tea

AOAC SPSFAM ERP REVIEW: MAIN FORM

Submission Date

2017-09-16 22:05:30

Name

Rasu Jayabalan

E-mail

jayabalanr@nitrkl.ac.in

Organization

National Institute of Technology, Rourkela, Odisha, India

Title of Method

Determination of alcohol content in kombucha tea by head space solid phase microextraction and gas chromatography-mass spectrometry

AOAC Candidate Method Number (e.g. ALN-01)

KOM-05

Applicable SMPR

Ethanol in Kombucha Tea (2016.001)

I. Summary of the Method

This is a head space - solid phase microextraction (HS-SPME) GC-MS developed for determination of alcohol in Kombucha tea. The method describes the reagent preparation, instrument details and validation of the method using GC-MS. Suitable standards and samples were used. Dilutions need to be followed are explained. The method detects and quantitate the ethanol within the prescribed limits of LOD and LOQ. However the role of Mass spectrometry is not clearly mentioned.

II. Review of the Method Only 1. Does the applicability of the method support the applicability of the SMPR? If not, please explain what is missing. 2. Does the analytical technique(s) used in the method meet the SMPR? If not, please specify how it differs from what is stated in the SMPR. 3. Are the definitions specified in the SMPR used and applied appropriately in the method? If no, please indicate how the terms are used. 4. Does the method, as written, contain all appropriate precautions and warnings related to the method's reagents, components, instrumentation, or method steps that may be hazardous? If no, please suggest wording or option(s).

Yes. The method has quoted the SMPR parameters whereever applicable.

Yes. The method meet the SMPR parameters like LOD, LOQ, and Reproducibility. All the parameters checked are within the prescribed limits given in SMPR.

Yes. The method has quoted the SMPR parameters and applied them appropriately to validate the method.

Ethanol, sodium chloride, sodium potassium phosphate (monobasic and dibasic) are used in the method. Hazardous reagents were not used in this method.