Expert Review Panel for Kombucha Tea

3. Is there information demonstrating that the method system suitability tests and controls as specified in the SMPR worked appropriately and as expected? If no, please specify. 4. Based on the supporting information, is the method written clearly and concisely? If no, please specify the needed revisions. 5. Based on the supporting information, what are the pros/strengths of the method? 6. Based on the supporting information, what are the cons/weaknesses of the method?

Yes. The method has utilized SMPR recomended 200 proof ethanol (99.5%) and Low alcohol beer (nominal 0.50% ABV) from Millipore Sigma as standard.

Yes. the method is written clearly and concisely.

Strengths: 1. The method is able to accurately detect the ethanol concentration as low as 0.03%.

Weakness: 1. Since the method needs GC-MS, small analytical laboratories will not be able to use the method. 2. Method needs highly skilled technicians. 3. Method is considered to be expensive due to the use of GC-MS. The method and results given in the manuscript is able to quantitate the ethanol as per SMPR specifications. The method used recommended standards and samples. The method explains the dilution protocol need to be followed. Skilled technicians would be able to follow the method clearly. Yes. I would recommend this method be adopted as a First Action and publish in the Official Methods of Analysis of AOAC. Rationale: The methodology was developed using standards and samples recommended by SMPR and is able to detect and quantitate ethanol level prescribed in the SMPR. The method performance requirements given in SMPR is applicable.

7. Any general comments about the method?

V. Final Recommendation Do you recommend this method be adopted as a First Action and published in the Official Methods of Analysis of AOAC INTERNATIONAL? Please specify rationale.