Expert Review Panel for Kombucha Tea

AOAC SPDS ERP REVIEW: MAIN FORM

Submission Date

2017-09-20 10:01:09

Name

Armen Mirzoian

E-mail

armen.mirzoian@ttb.gov

Organization

TTB

Title of Method

Enzytech Liquid Ethanol

AOAC Candidate Method Number (e.g. ALN-01)

KOM-07

Applicable SMPR

2016.001

Summary:

This is an enzymatic method for determination of low level ethanol content in kombucha, fruit juices and alcohol-free beer. The quantification is based on catalytic activity of alcohol dehydrogenase which oxidizes ethanol to acetyldehyde and converts NAD to NADH. Absorbance NADH is measured at 340 nm and is converted to ethanol concentration.

1. Does the applicability of the method support the applicability of the SMPR? If not, please explain what is missing. 2. Does the analytical technique(s) used in the method meet the SMPR? If not, please specify how it differs from what is stated in the SMPR. 3. Are the definitions specified in the SMPR used and applied appropriately in the method? If no, please indicate how the terms are used. 4. Does the method, as written, contain all appropriate precautions and warnings related to the method's reagents, components, instrumentation, or method steps that may be hazardous? If no, please suggest wording or option(s).

Yes. The method measure alcohol content of kombucha in the applicable range.

Yes, it does.

They do. Alcohol concentrations are expressed thought the text on g/L bases. It might be useful to also express them in %abv bases next to g/L.

yes.