Expert Review Panel for Kombucha Tea

AOAC SPDS ERP REVIEW: MAIN FORM

Submission Date

2017-09-14 10:05:24

Name

Kathy Stenerson

E-mail

katherine.stenerson@sial.com

Organization

MILLIPORESIGMA

Title of Method

The Determination of Low Level Ethanol in Kombucha

AOAC Candidate Method Number (e.g. ALN-01)

KOM-08

Applicable SMPR

2016.001

Summary:

This method is the headspace GC/MS determination of ethanol in Kombucha tea. The sample is diluted 100X, n-butanol added as internal standard, and analyzed against a calibration curve prepared in water. The method does determine low levels of alcohol in Kombucha, however it does not express the values obtained as % ABV. The actual values calculated are % alcohol by weight since calibration is for ug of ethanol.

1. Does the applicability of the method support the applicability of the SMPR? If not, please explain what is missing. 2. Does the analytical technique(s) used in the method meet the SMPR? If not, please specify how it differs from what is stated in the SMPR. 3. Are the definitions specified in the SMPR used and applied appropriately in the method? If no, please indicate how the terms are used. 4. Does the method, as written, contain all appropriate precautions and warnings related to the method's reagents, components, instrumentation, or method steps that may be hazardous? If no, please suggest wording or option(s).

The analytical technique of this method (GC-MS) adequately met the SMPRs of accuracy and repeatability for the spike levels done. Due to the calculation by weight for ethanol, the lower spike levels necessary to demonstrate applicability at 0.1% ABV and the LOQ of .05% ABV were not truly demonstrated, however it is felt that the method would meet these requirements.

%ABV was not appropriately applied, as values reported are actually percent alcohol by weight. All other definitions are adequately used.

The method as written does not contain precautions related to the flammability and toxicity of butanol and ethanol. Suggested addition: Ethanol and butanol are flammable solvents, keep away from ignition sources and flames. During use, avoid skin contact, wear eye protection, and avoid breathing vapors by working under a fume hood if possible.