FAM ERP 11-9-21 (OMB Review)

EXPERT REVIEW PANEL Food Authenticity Methods (FAM) DOCUMENTS REVIEW

Tuesday, November 9, 2021

AOAC INTERNATIONAL 2275 Research Blvd. Ste. 300 Rockville, MD, 20850 UNITED STATES

dboyd@aoac.org 301.924.7077 x126

MEMORANDUM

DATE:

November 9, 2021

TO:

AOAC Official Methods Board (OMB)

FROM: Delia Boyd, Senior Manager SUBJECT: AOAC Food Authenticity Methods (FAM) Expert Review Panel (ERP)

Background: In accordance with the policy for Official First Action, an expert review panel is being assembled toreview the methods submitted into the AOAC FAM process for priority nutrients. AOAC staff has collected CVs and they are on file at AOAC in accordance with the revised ERP policiesand procedures. A proposal for the AOAC FAM Expert Review Panel is submitted for your consideration.

The attached package contains the following information: CVs for all proposed candidate(s)

 List of Expert Review Panel (ERP) members  Balance of Perspectives  CVs

This expert review panel operates under AOAC policies and procedures. Each expert is required to sign the AOAC Volunteer Acceptance Form which includes adherence to the AOAC policy for Volunteer Conflict of Interest, Antitrust and Use of Association Name, Letterhead and Logo. These documents will be enforced by the Expert Review Panel chair and facilitated by AOAC staff. We request the OMB to review and confirm the expertise of proposed candidates also reviewing the balance of the panel as well as the conflicts of interest of proposed members. This Expert Review Panel is scheduled to meet and review validation plans. Your review and approval of the panel is requested. Please address questions regarding the attached package to me and thank you for your consideration.

AOAC INTERNATIONAL FOOD AUTHENTICITY METHOD (FAM) EVOO/Honey/Milk Expert Review Panel (ERP)

Recommendation: Names for review and vetting the AOAC FAM Expert Review Panel (ERP) – EVOO/Honey/Milk NON-TARGETED TESTING

1. Sneh Bhandari 2. Sachin Chaudhari 3. Per Waaben Hansen 4. Govind Raj Joshi 5. Robert LaBudde 6. Salvatore Parisi 7. Amr Fuad Saeed 8. Nasiruddin Shaikh

Independent. Consultant

CSIR-CFTRI

FOSS Analytical A/S

Dairy Development Corporation Least Cost Formulations, Ltd. Lourdes Matha Institute HMCT National Dairy and Food -HSA

ICAR-National Research Center for Grapes, Pune

9. Mei Shotts 10. John Szpylka 11. Fenfen Tang 12. Kenny Xie

Abbott

Food Safety Net Services The Ohio state university

US Pharmacopeia

13. Hong You

Eurofins

TARGETED TESTING

1. Sneh Bhandari 2. Sachin Chaudhari 3. Ajai Prakash Gupta 4. Per Waaben Hansen 5. Govind Raj Joshi 6. Kevin Kubachka 7. Robert LaBudde 8. Salvatore Parisi 9. Amr Fuad Saeed 10. Nasiruddin Shaikh

Independent. Consultant

CSIR-CFTRI CSIR-IIIM

FOSS Analytical A/S

Dairy Development Corporation

US FDA

Least Cost Formulations, Ltd. Lourdes Matha Institute HMCT National Dairy and Food -HSA

ICAR-National Research Center for Grapes, Pune

11. John Szpylka 12. Fenfen Tang 13. Kenny Xie

Food Safety Net Services The Ohio state university

US Pharmacopeia

14. Hong You

Eurofins

AOAC INTERNATIONAL FOOD AUTHENTICITY METHOD (FAM) EVOO/Honey/Milk Expert Review Panel (ERP)

Statement of Expertise:

FIRST NAME

LAST NAME

ORGANIZATION

EXPERTISE

Sneh

Bhandari

Independent Consultant

Research Experience in Milk authentication and milk adulteration: Involved in research in milk authentication and creating standards for this purpose for over 10 years. Was chair of USP sub panel on creating Amino acids as a tool to establish authenticity of skim milk powder. The USP monograph on skim milk powder authentication using amino acid fingerprinting as a tool is to be published year based on that work. Currently I am chair of Expert Panel at USP Dietary protein with a goal to establish standards for authentication of premium value food proteins traded in high volume. The panel has completed work in creating tools for whey authentication including amino acids, peptide fingerprinting, and intact protein scanning by LC-MS. Some of these methodologies for whey authentication are being published as FCC methodologies this year. •More than 33 years of experience in the area of Analytical Chemistry of Natural Products and Pharma post M. Sc. •Expertise in method development, validation & analysis of tissue culture samples and bioactive constitutions of microbes and medicinal plants using sophisticated equipments like LC/MS/MS, UPLC, HPTLC, LC-MS-TOF, GC-MS and HPLC. My area of expertise also includes pharmo-kinetics & pharmo-dynamics (PKPD) samples, drug metabolites, mechanistic study using UPLC/HPLC and LC/MS/MS. •Jammu & Kashmir FDA approved Quality Control person for Herbals and Phama drugs. •Published 110 research papers in journals of National and International repute and 11 book chapters. Expertise in Spices and application of NMR in food quality

Sachin

Chaudhari

CSIR-CFTRI

Ajai Prakash

Gupta

CSIR-IIIM

•Delivered 32 invited lectures nationally and internationally. •Attended 26 conferences nationally and internationally. •Published papers were acknowledged through ten awards. •Three national/International patents.

Per Waaben

Hansen

FOSS Analytical A/S

I will be able to contribute with my 25+ years experience with developing equipment for food analysis, in particular: - Data analysis (chemometrics) focusing on targeted and untargeted screening for adulterants in milk

- Near infrared spectroscopy - Mid infrared spectroscopy

Govind Raj

Joshi

Dairy Development Corporation

I am working as Head of Department of Quality Control & Technology development in Dairy Development Corporation, Government of Nepal fully owned industry. I am a dairy/food technologist and working there for nine years. I have been using isotope ratio analysis for food adulteration, much of which has focused on addition of low-cost sweeteners to honey, for the last 10 years and am one of the primary such analysts/experts at the Food and Drug Administration. I can provide relevant publications and presentations if necessary.

Kevin

Kubachka

US FDA

Robert

LaBudde

Least Cost Formulations, Ltd.

Chemistry, Chemometrics, Statistics

Salvatore

Parisi

Lourdes Matha Institute HMCT

I have various experiences in AOAC. With relation to food authenticity, I am currently member of: a) AOAC Food Authenticity/Fraud Working Group (Targeted) b) AOAC Food Authenticity/Fraud Working Group (Non-Targeted). In addition, I am an OMB Member. Finally, I have published something concerning food authenticity so far: Haddad, M. A., Yamani, M. I., Da’san MM, J., Obeidat, M., Abu-Romman, S. M., & Parisi, S. (2021). Dried Fermented Dairy Products in Jordan: Jameed and Traceability. In Food Traceability in Jordan (pp. 53-63). Springer, Cham. 50. Al-Dalain SY, Haddad MA, Parisi S, Al-Tarawneh MA, Qaralleh H (2020). Determination of Macroelements, Transition Elements, and Anionic Contents of Commercial Roasted Ground Coffee Available in Jordanian Markets. BEVERAGES, ISSN: 2306-5710, doi: 10.3390/beverages6010016 Parisi, S., Varghese, S. M,, Parisi, C. (2021) The HITS Strategy and Chemometrics. Agro FOOD Industry Hi Tech 32, 1:58-60 I have extensive experience working with International Medical Corps as a Field Monitor. I am adept at solving field issues practiced at documenting data and able to handle the physical demands of a field assistant. In my current role I consistently record data accurately enabling other departments to complete their roles on time. Field monitoring and verification visits conducted to ensure that all activities are implemented according to the project implementation plans. Also, I worked as Research Assistance at IBB University. So, this position gave me a strong background in the Nutrition and Food Science with special expertise in chemical and Microbial laboratory for Water and Food tests I am currently developing targeted and non-targeted analytical methods for food authenticity/adulteration testing in pomegranate juice, grape juice and ayurvedic herbal products using LC-HRMS. Chair of the FAM Non-Targeted Testing Working Group. Past chair AOAC OMB. Experienced in testing both targeted and non-targeted methods for foods notably vitamins, carbohydrates and contaminants. I received my bachelor's degree in food quality and safety in 2015. Then I worked in pesticide residues in fruit and vegetable inspection lab for two years, which allowed me to learn and have hands-on experience with standard methods of pesticide detection using chromatography and Mass spectrometry. I also participated in method development and validation for pesticide residue limit standards of new agricultural produce. I started graduate school in 2018. My research focuses on NMR-based metabolomics for food quality and authenticity evaluation. I have used various 1D and 2D NMR methods for compositional profiling and quantification of pomegranate juice, olive oil, and avocado oil samples. I applied NMR with supervised (OPLS-DA, random forest, XGBoost) or unsupervised (PCA) models for authentication of PJ, avocado oil, and botanical origin discrimination of PJ and olive oil. I also conducted comparison studies for quantification agreement evaluation between NMR with a traditional or standard analytical instrument like GC. I am experienced with experimental designing, univariate data analysis, and multivariate data analysis using multiple platforms (SIMCA, R, matlab, SPSS, Jmp). Dr. Hong You is a Director of Scientific Affairs at Eurofins, the largest contract TIC (Testing, inspection and certification) corporation worldwide by revenue and profits. He oversees the company’s Research & Development activities and leads senior (Ph.D.) scientists to conduct analytical method development/validation/harmonization projects for testing dietary supplements, botanicals, sports nutrition products, and functional foods. Dr. You is a Technical Working Group Member (Chemical Analysis) of the US FDA Botanical Safety Consortium. He is also a member of the AOAC Analytical Solutions Forum Steering Committee, a leadership team member of the Institute of Food Technologists - Nutraceuticals and Functional Foods (IFT NFF) Division, an Expert FCC SL Worked in non-targeted methods for the past 15 years

Amr Fuad

Saeed

National Dairy and Food - HSA

Nasiruddin

Shaikh

ICAR-National Research Center for Grapes, Pune

Mei

Shotts

Abbott Nutrition

John

Szpylka

Food Safety Net Services

Fenfen

Tang

The Ohio State University

Kenny

Xie

US Pharmacopeia

Hong You

You

Eurofins

Review Panel member for AOAC Official Methods of Analysis (OMA), and an Editorial Board Member of Journal of Food Biochemistry. Dr. You has published more than 20 peer-reviewed papers on top-tier academic journals including Journal of Agricultural and Food Chemistry, Food Chemistry, Talanta, J of AOAC Int., etc. and has reviewed more than 150 manuscripts and meeting abstracts for the American Botanical Council Botanical Adulterants Prevention Program (ABC BAPP), Journal of Agricultural and Food Chemistry, Food Chemistry, Food Research International, Phytochemical Analysis, J of AOAC Int, J Food Biochemistry, and American Society for Nutrition (ASN) and IFT Annual Conferences. He was invited to serve as session chairs and give presentations at scientific conferences, trade shows, and universities.

AOAC INTERNATIONAL FOOD AUTHENTICITY METHOD (FAM) EVOO/Honey/Milk Expert Review Panel (ERP)

Balance of Perspective (Regional):

FIRST NAME

LAST NAME

ORGANIZATION

BROAD PERSPECTIVE

REGION

Sneh

Bhandari Chaudhari

Independent Consultant

Industry

US US

Sachin

CSIR-CFTRI

Government

Ajai Prakash

Gupta

CSIR-IIIM

Government

US

Per Waaben

Hansen

FOSS Analytical A/S

Industry

Denmark

Govind Raj

Joshi

Dairy Development Corporation

Industry

US US

Kevin

Kubachka

US FDA

Government

Robert

LaBudde

Least Cost Formulations, Ltd.

Academia

US

Salvatore Amr Fuad Nasiruddin

Parisi Saeed Shaikh

Lourdes Matha Institute HMCT National Dairy and Food - HSA ICAR-National Research Center for Grapes, Pune

Academia

US

CRO CRO

Yemen

India

Mei

Shotts

Abbott Nutrition

Industry Industry Academia

US US US US

John

Szpylka

Food Safety Net Services The Ohio State University

Fenfen Kenny

Tang

Xie

US Pharmacopeia

Organization

AOAC INTERNATIONAL FOOD AUTHENTICITY METHOD (FAM) EVOO/Honey/Milk Expert Review Panel (ERP)

Balance of Perspective (Regional):

AOAC INTERNATIONAL FOOD AUTHENTICITY METHOD (FAM) EVOO/Honey/Milk Expert Review Panel (ERP)

Balance of Perspective (Specific):

3410 Beckwith Lane, Crete, IL 0417 Phone: 708 843-2694

Sneh Bhandari

Research Experience

Research Experience in Milk authentication and milk adulteration: Involved in research in milk authentication and creating standards for this purpose for over 10 years. Was chair of USP sub panel on creating Amino acids as a tool to establish authenticity of skim milk powder. The USP monograph on skim milk powder authentication using amino acid fingerprinting as a tool is to be published year based on that work. Currently I am chair of Expert Panel at USP Dietary protein with a goal to establish standards for authentication of premium value food proteins traded in high volume. The panel has completed work in creating tools for whey authentication including amino acids, peptide fingerprinting, and intact protein scanning by LC-MS. Some of these methodologies for whey authentication are being published as FCC methodologies this year. SDB Consultancy, Crete, IL Provide consultancy service to food industry on variety analytical issues related to nutrition labeling, establishing authentication or adulterated food matrices and related issues. February 2020 – till now • Direct method development in food science and nutritional analysis employing various analytical platforms including LC-MS, GC-MS, LC, GC, SPE, ELISA, ICP-MS, enzyme based assays, etc. • Manage R&D Dept. including its budget, development, growth & business plans. • Manage routine analysis performed in the department under strict quality parameters under ISO 17025 and other quality parameters. • Prepare SOPs, Quality documents and perform all activities to comply with ISO 17025 requirements. Transfer new methods, policies and protocols in operation working in ISO 17025 environment. • Manage food industry client’s research and development projects. • Developed a LC-MS/MS method of folate analysis (AOAC Final Action Method 2011.06) which is approved as a CODEX type II method of folate analysis in food. • Serves as a technical resource to the Silliker’s food industry clients regarding their analytical and technical issues related consumer complain, product failure, nutrition labeling issue, verification of nutritional claim, quality issues and compliance to regulatory guidelines and target specifications. • Develop SOPs, Policies and the method protocols including quality management for routine operation. • Work with National & International bodies to lead efforts in improving methods of food, May 19, 1997 – Jan 31, 2020 60417 Research and Development Director Merieuex NutriSciences/Silliker, Crete, IL

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dietary supplement analysis. • Fellow of AOAC. Expert review panel & strategic committee member for AOAC SPIFAN program. • Chair AOCS Analytical Division. • Member Chromatography Division, AOCS • Help clients about regulatory guidelines (FDA, USDA) including nutritional labeling. • Wrote a chapter on food hazards resulting from environmental, industrial and agricultural contaminants. • Made various presentations at professional and scientific meetings including AOAC, IFT, AOCS etc. • Helped clients to study oil authenticity and oil adulteration. • Served on various AOAC Expert Review Panels and Stakeholder Panels. • AOAC Technical Committee member for Additives (2004-2007). • Was involved in EPR for vitamin E analysis in dietary supplements (AOAC task force; FDA/NIH). • Validated HPLC/GC Methods to analyze various nutritionally important food analytes, i.e. organic acids. Preservatives, tocopherols, tocotrienols, water soluble vitamins, fat soluble vitamins, , nutraceuticals, capsaicin, cholesterol & phytosterols, sugars, sugar alcohols etc. July 1, 1987– March 31, 1990 Dept of Food Science & HN, Gainesville, FL Postdoctoral Research Associate • Metabolism and bioavailabilty of folate vitamers in humans using stable isotopes & mass- spec. April 4, 1990 – May 18, 1997 Silliker LaboratoriesChicago Heights, IL • Research Manager; Method Development Specialist

Research Biochemistry

Feb 10, 1986 – June 31, 1987

Dept of Pharmacology, LSU Med. Ctr, Shreveport, LA

Postdoctoral Research Fellow • Folate bioavailability, metabolism and its transport in intestinal brush border

1982–1983

Dept. of Pediatrics, AHSC, Tucson, AZ

UNESCO Postdoctoral Research Fellow • In vitro studies on sugar transport using everted gut sac technique and its correlation with • activities of digestive enzymes. • Studies on thyroid hormone receptors in isolated intestinal epithelial crypt and villi cells.

1977–1980

Biochemistry Dept., MS University, Baroda, India

Principal Investigator • Studies on intestinal phosphoinositide metabolism.

1972–1976

Biochemistry Dept., MS University, Baroda, India

Research Fellow

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• Ph.D. Thesis: Studies on intestinal enzymes and biochemistry in relation to nutrition .

1972–1972

Biochemistry Dept., MS University, Baroda, India

Research Fellow • Studies on soybean hemagglutinins & trypsin inhibitors , effect of food processing on the same. 1984 – 1986 Reader (Associate Professor) of Biochemistry, MS University of Baroda, Vadodara, India: Taught Biochemistry, Human Physiology & Clinical Chemistry, Cell Biology, Cell Metabolism, Plant Biochemistry, Human Nutrition and Food Chemistry to post-graduate students . 1976 – 1984 Lecturer (Assistant Professor) of Biochemistry MS University of Baroda, Vadodara, India: Taught Biochemistry, Human Physiology & Clinical Chemistry, Cell Biology, Cell Metabolism, Plant Biochemistry, Human Nutrition and Food Chemistry to post-graduate students .

Teaching Experience

Education

1972–1977

Biochemistry Dept., MS University, Baroda, India

• Ph.D. in Biochemistry (Nutrition)

1970–1972

Biochemistry Dept., MS University, Baroda, India

• M.S. in Biochemistry. Specialization: Human Nutrition.

C.N.S.

1994

Certification Board for Nutr. Sp., American College of Nutrition

• Certified Nutrition Specialist (C.N.S.) •

♦ Member of AOAC SPIFAN ERP & Stakeholder Panel ♦ Chair AOCS Analytical Division, 2012; Vice Chair AOCS Analytical Division, 2012 ♦ Fellow of AOAC International, 2011 ♦ Member of the AOAC Official Methods Board since 2010 - 2016 ♦ Involved in AOCS in Chromatography Technical Committee ♦ AOAC/FDA/NIH Expert Review Panel member – Coenzyme Q (2005) ♦ Member of the Vitamin E Analysis in Dietary Supplements for AOAC/FDA/NIH Task force. ♦ AOAC Associate Referee for Vitamins. ♦ Listed in Strathmore’s Who’s Who Directory (1995-96). ♦ UNESCO Postdoctoral Fellowship (1982).

Honors

Publications

♦ Original Research Papers Published: 21

♦ Book Chapter

:

1 (In: Handbook of Food Safety, 2003, Wiley)

♦ Presentations at Scientific meetings : > 150

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Curriculum Vitae __________________________________________________________________________________________

Dr. Sachin R. Chaudhari Scientist and Assistance Professor, Department of Spice and Flavour Science, CSIR-Central for Food Science and Technological Institute, Mysore, India.

E-mail : aailasachin@gmail.com sachinnmr@cftri.res.in Phone: +919606350169

Research Interests

Methods development in solution and solid state NMR and their applications to the food science world. The goals of my research are to highlight advances in NMR spectroscopy and provide insight into how new methods can be deployed to address a number of important measurement challenges and study a range of food materials and pharmaceuticals both a solution- and solid-state NMR perspectives. Education __________________________________________________________________________________________

Degree

Subject

University/Institution

Duration

Ph.D.

Chemical Science (Core area: NMR spectroscopy)

NMR Research Centre, Indian Institute of Science, Bangalore, India. Department of Chemistry, Nagpur University, Maharashtra, India. Sardar Patel College, Chandrapur, Nagpur University, Maharashtra, India.

2009-2014

M.Sc.

Chemistry

2006 – 2008

B.Sc.

Phys, Chem & Math

2003 - 2006

Current Position __________________________________________________________________________________________ Scientist, Department of Spice and Flavour Science, CSIR-Central for Food Science and Technological Institute, Mysore, India. Previous Position __________________________________________________________________________________________ 1. Research Associate, NMR Research Centre, Indian Institute of Science, Bangalore, India, Janaury- 2014 -August- 2014 , under the guidance of Prof. N. Suryaprakash. 2. Post-doctoral Fellow, Centre de RMN Très Hauts Champs, Université de Lyon (CNRS/ENS Lyon/UCB Lyon 1) 69100 Villeurbanne, France, September- 2014 to August- 2016 , in the group of Prof. Lyndon Emsley and Dr Anne Lesage. 3. Post-doctoral Fellow, Laboratory of Magnetic Resonance and Modeling and Exploration of Materials, INAC, CEA and Grenoble Alpes University, 17, rue des Martyrs, Grenoble, France, September- 2016 to April- 2017 in the group of Dr. Gael De Paepe. 4. Marie Skłodowska -Curie Fellow, School of Chemistry, University of Nottingham, University Park, Nottingham, NG7 2RD, UK, 2017-2018 , in the group of Prof. Jeremy Titman. Fellowship and Academic Achievements __________________________________________________________________________________________ 1. Qualified the Council of Scientific and Industrial Research- National Eligibility Test

(CSIR-NET), India for Junior Research Fellowship and Eligibility for Lectureship, June- 2009. 2. Qualified the Maharashtra State Eligibility Test (MH-SET), India for Assistant Professorship Conducted by University of Pune as a State agency, Maharashtra, India, 2009. 3. Qualified the GATE (Graduate Aptitude Test of Engineering, India) in 2010. 4. Awarded CBMR-NMRS Gold Medal award for Excellence in Research in India (National Level) in the field of NMR Spectroscopy for the Year- 2012. 5. Awarded the Jharana Rani Samuel, Best Student Award for Excellence in Research in India (National Level) in the field of NMR Spectroscopy for the Year- 2013. 6. Awarded CBMR-NMRS Gold Medal award for Excellence in Research in India (National Level) in the field of NMR Spectroscopy for the Year- 2014. 7. Selected Marie-Curie Individual Fellowship for postdoctoral research funding from the Marie- Skłodo wska-Curie Actions- 2016. Grant Received 1) Grant: Grant-in-Aid, Name of Funding Organization: Science and Engineering Research Board (SERB), India, Amount Awarded : 30 lakh, Period of Grant Award: Sept- 2019-Oct-2021, Title of Project : Development and Applications of NMR methods for Qualitative and Quantitative analysis of Curcuminoids, Role on Project: PI. 2) Grant: CSIR-Mission Mode, Name of Funding Organization: CSIR-India, Amount Awarded : 4.21 Crore, Period of Grant Award: Oct-2018-Oct-2020, Title of Project : Food and Consumer Safety Solutions, Role on Project: CO-PI. 3) Grant: Grant-in-Aid, Name of Funding Organization: Coconut Development Board, Government ministry, Amount Awarded : 27.50 lakh, Period of Grant Award: Oct-2018- Oct-2020, Title of Project : Design and Development of Reactor for Processing of Coconut-Based Beverages with UV-C Irradiation, Role on Project: CO-PI. 4) Grant: Sponsored Project, Name of Funding Organization: Lakshmi Srinivasan Private limited, Bangalore, Amount Awarded : 10.5 lakh, Period of Grant Award: May-2019- Jan-2020, Title of Project :Development of hygienic technology for preparation of Herbal/spice water, Role on Project: CO-PI. 5) Grant: Sponsored Project, Name of Funding Organization: Prestige limited, Hosur, Tamilnadu, India, Amount Awarded : 4.0 lakh, Period of Grant Award: June-2019-Dec- 2018, Title of Project : Leaching of heavy metals from stainless steel coupons/cookware in food simulants and food materials, Role on Project: CO-PI. 6) Grant: Individual Fellowship, Name of Funding Organization: Marie- Skłodowska -Curie Actions-, Europe, Amount Awarded : Fellowship, Period of Grant Award: May-2018-Oct- 2020, Title of Project :, Role on Project: PI.

Teaching and Guidance

Composition Instructor 

Planned and taught an intensive course on NMR spectroscopy.  Demonstration of CFTRI technologies and training session to farmer and industrials.  Taught in part with an innovative, interdisciplinary team-teaching program design short term course on various aspects. Teaching Assistance  Facilitator for NMR section of Analytical techniques.  Taught a section on NMR spectroscopy, theory and practical’s  Planned lessons and assignments, led discussion sections, graded papers and exams.

Guidance & Supervision Ph.D. Number of Ph.D. Student currently working: 1 and Project assistance: 1 Dissertations students April 2010-August 2010: Supervised one graduate student. April 2012-August 2012: Supervised two M.Sc. students. April 2013-August 2013: Supervised one post graduate (M.Pharm.) student and junior college lecture. April 2014-August 2014: Supervised three M.Sc. and two under graduate students. August-2017-December-2017: Supervised two Master Students Feb-2017-: Supervised two Dissertation Students December-2018: Supervised one Dissertation Students Presentations at Conferences and Symposia __________________________________________________________________________________________ 1. Poster presented at the 17 th Annual NMRS ( National Magnetic Resonance Society) symposium, Guru Nanak Dev University, Amritsar, India, 2011 . 2. Attended workshop on Recent Advances in NMR Spectroscopy, organised by Tata Institute of Fundamental Research (TIFR), Hyderabad, India, 2011 . 3. Oral presentation at the Indo-Swiss Symposium on Recent Trends in NMR of Biomolecules and Advanced Materials, NCL (National Chemical Laboratory), Pune, India, 2012. 4. Poster presented at the 18 th Annual NMRS (National Magnetic Resonance Society) symposium, Indian Institute of Science, Bangalore, India 2012 . 5. Poster presented at the International conference EUROMAR-2012 (European Magnetic Society) held at the University of Dublin, Dublin, Ireland, 2012 . 6. Oral presentation at the 1 9 th Annual NMRS (National Magnetic Resonance Society) Symposium, organised by TIFR and Indian Institute of Chemical Technology, Mumbai, India, 2013 . 7. Poster presented at the 1 9 th Annual NMRS (National Magnetic Resonance Society) Symposium, Organised by TIFR and Indian Institute of Chemical Technology, Mumbai, India 2013 . 8. Poster presented at SMASH-2013 (Small molecule NMR conference) at Santiago the Compostela, Spain, 2013 . 9. Attended workshop on the Ultrafast NMR held at the Santiago de Compostela, Spain , 2013 . 10. Oral Presentation at the NCC-2013 (National Conference on Chirality) + Symposium, organised by M. S. University of Baroda, Baroda, India, 2013 . 11. Invited guest lecture at the Government Science college for Women, Hindupur, Andhra Pradesh, India, December- 2013. 12. Oral presentation at the 20 th Annual NMRS (National Magnetic Resonance Society) symposium, organised by Tezpur University, Tezpur, India, 2014 . 13. Oral presentation at the 21 th Annual NMRS (National Magnetic Resonance Society) symposium, organised by, Guru Nanak Dev University, India, 2015 . 14. Poster presented at the Eu-Cost Summer School On Nuclear Spin Hyperpolarization Techniques, organised by University of Southampton, UK and Goethe University Frankfurt, Southampton, UK, 2015 . 15. Poster presented at 9 th the Alpine Conference on Solid State, NMR, organised under the auspices of the groupement Ampere and the International Society of Magnetic Resonance, Chamonix-Mont Blanc, France, 2015 . 16. Poster presented at 58 th Annual Rocky Mountain Conference on Magnetic Resonance in Breckenridge, Colorado which endorsed by American Chemical and Applied Spectroscopy Society, 2016 .

17. Presented talk on Dynamic Nuclear Polarization under fast magic angle spinning conference 7th Asia Pacific NMR Conference (APNMR-2017) held in IISc, Bangalore, 2017 . 18. Presented talk on solvent suppression in MAS DNP meeting at Catalyst design by NMR, Lille, France, March- 2017 . 19. Oral presentation at IUPAC- 2017 , held at Hyderabad, India. Invited Talks 1. Delivered talk at Mahatma Gandhi Senior Science College, Nagbhid, Maharashtra, India, 2014 under Inspire programme to graduate/postgraduate students . 2. Delivered talk at Nevajabai Hitkarni Senior Science College, Bramhapuri, Maharashtra, India, 2016, under Inspire programme to graduate/postgraduate students . 3. Delivered tutorial lectures at the workshop on solid state NMR for materials and biomolecules organised by Institute of Organic Chemistry, Riga, Latvia, 2016 . 4. Delivered talk at Indian Institute of Science and Education Research, Mohali, Punjab, India. 21 st August, 2016 on DNP Enhanced solid state NMR: The development and applications. 5. Departmental Seminar, (On Developments and Applications of New Methods for solution NMR & DNP Enhanced solid State NMR), IIT, Roorkee, August-24- 2016. 6. Departmental Seminar, (On Developments and Applications of New Methods for solution NMR & DNP Enhanced solid State NMR), IIT, Ropar, August-29- 2016. 7. Departmental Seminar, (On Developments and Applications of New Methods for solution NMR & DNP Enhanced solid State NMR), IIT, Mumbai, September-2- 2016. 8. Departmental Seminar, (On Developments and Applications of New Methods for solution NMR & DNP Enhanced solid State NMR), IIT, Patna, Jan-2- 2017. 9. Departmental Seminar, (On Developments and Applications of New Methods for solution NMR & DNP Enhanced solid State NMR), IIT, Bhubaneshwar, Feb-10- 2017. 10. Delivered talk at Sardar Patel Mahavidyalaya, Chandrapur, Maharashtra, India, 2017, under Inspire programme to graduate/postgraduate students by Alumni. 11. Invited talk in the conference 7th Asia Pacific NMR Conference (APNMR-2017) held in IISc, Bangalore, 2017 . 12. Invited talk in the conference 5th Annual Conference of AnalytiX 2017 (AnalytiX- 2017) Fukuoka, Japan, 2017 . 13. Invited talk in the 6th Annual World Congress of Advanced Materials- 2017 (WCAM- 2017, Xi’an) Xi’an China, 2017 . 14. Invited Chair/Speakers in the 8th World Gene Convention-2017, Macao, China during November 13-15, 2017. 15. Oral Speech Invited in the 15th Annual Congress of International Drug Discovery Science and Technology - Japan 2017 (IDDST-Japan 2017) held during July 25-27, 2017 in the city of Osaka in Japan. 16. Departmental Seminar, (On Developments and Applications of New Methods for solution NMR & DNP Enhanced solid State NMR), IIT, Indore, August-08- 2017. 17. Delivered a guest lecture in Faculty development programme “Characterization of Materials by Advanced Analytical Techniques” organised by JSS College, Mysuru, 21 st June 2019 . 18. Delivered a seminar in the Training Program theme on "Scientific Equipment’s: Application and Data Analysis in Advanced Research" organised by University of Mysuru, Mysuru, 20 th Sept. 2019 . Membership in Scientific Societies 1. Life member, National Magnetic Resonance Society of India , India . 2. Life member, Association of Food Scientists & Technologists India, India.

RESUME

Dr. Ajai Prakash Gupta

Principal Technical Officer & Associate Professor Academy of AcSIR, Drug Testing Laboratory (QMI), CSIR-Indian Institute of Integrative Medicine, Canal Road, Jammu-180001 Date of Birth: 05 th June 1968. Phones: +919469211528, +917006146311;(Mobile); +91191-2585000-09 (Ext. 341) (Office); Fax: +91191-2586333; Email: ajai747@yahoo.co.in; ajai747@gmail.com; apgupta@iiim.res.in Expertise • More than 33 years of experience in the area of Analytical Chemistry of Natural Products and Pharma post M. Sc. . • Expertise in method development, validation & analysis of tissue culture samples and bioactive constitutions of microbes and medicinal plants using sophisticated equipments like LC/MS/MS, UPLC, HPTLC, LC-MS-TOF, GC-MS and HPLC. My area of expertise also includes pharmo-kinetics & pharmo-dynamics (PKPD) samples, drug metabolites, mechanistic study using UPLC/HPLC and LC/MS/MS. • Jammu & Kashmir FDA approved Quality Control person for Herbals and Phama drugs. • Published 110 research papers in journals of National and International repute and 11 book chapters. • Delivered 32 invited lectures nationally and internationally. • Attended 26 conferences nationally and internationally. • Published papers were acknowledged through ten awards. • Three national/International patents. • Library Chairman for three years. • Public Information Officer (PIO) for four years. • Secretary IIIM Café for three years. • IIIM Staff Club General Secretary for four years. • Technical expert committee members of High end equipments, Central University of Punjab (Bhatinda). • Technical expert committee members of High end equipments, Cultivation of Aromatic plants, (Uttarakhand Govt.) Dehradun. • Selected as Technical Expert Committee member of IPC, India. • NABL Assessor (ID number): 2271 . Experience in the Area of NABL and ISO certifications • Diploma in “ Total Q ality Management & ISO 9000 ”, (1997), (Correspondence), All India Institute of Management, Madras. Attended Conferences, Workshops & ISO training • Awareness Programme on ISO 9001:2000 QMS ”, On 5 th April 2004 at IHBT, Palampur (HP). • Internal Auditors Course on ISO 17025:1999 QMS ”, On 13 th July 2005 at IHBT, Palampur • National conference on Good Laboratory Practice (GLP) ”, at Industrial Toxicological Research Center, Lucknow, Jan 13-15, 2005. • Workshop on “ Validation of Analytical methods FDA and EU ”,13 th October 2011, Anacon, Mumbai . • Technical Manager of NABL in IHBT, Palampur for 3 years.

PROFILE

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2 • Analysis of Morphine, Codeine, Thebaine, Papaverine and Narcotine. Worked on projects to improve the efficiency of production plant and recovery of valuable alkaloids in process house. • Development of process of manufacturing value-added synthetic derivatives of morphine, codeine and thebaine viz. Oxycodone, Hydrocodone, Hydromorphone, Pholcodeine, Dihydro-codeine, Dihydro-morphine etc. • Member of team for NABL accreditation in CIMAP, Lucknow, IHBT, Palampur & IIIM, Jammu. • 4 days training on “ ISO/IEC-17025:2017-General Requirements for the Competence of Testing & Calibration Laboratories ”, 8-11 Feb-2021. • 2 days training on “ISO STANDARDS ON RISK MANAGEMENT (ISO 31000:2018 AND ISO 31010: 2019) ”, online. Organized by Quality Council of India. • AOAC Method Validation Training Course, 7-8 July 2021. EDUCATION 1998 Ph. D.: Specialized inAnalytical Chemistry: Central Institute of Medicinal & Aromatic Plants, (CSIR), Lucknow (R. M. L. Uni., Faizabad) 1989 1988 M. Sc.: Organic Chemistry; Kanpur University. Additional Qualifications: Post Graduate Diploma in Computer Application, Kamla Nehru Institute of Technology, Sultanpur. 1995 Bachelor of Education, Math & Science, Kanpur University. 1997 Diploma in “Total Quality Management & ISO 9000”, (Correspondence), All India Institute of Management, Madras. EXPERIENCE 2017-till date Senior Technical Officer-III, Drug Testing Laboratory ( QCQA) Presently I am engaged in the analysis of raw material, tablets, capsule from Pharma using HPLC, UPLC, GC-MS, Dissolution, Disintegration, TOC, Liquid Particle counter, Karl-fisher etc. I am also engaged in the analysis of bioactive constitution’s of tissue culture based sample, microbes and medicinal plants through HPLC, UPLC, GC-MS and LC-MS/MS. Senior Technical Officer-III, Quality Control & Quality Assurance I engaged in the analysis of PKPD samples, drug metabolites, mechanistic study, tissue culture based analysis and bioactive constitution’s of microbes, PKPD and medicinal plants through HPLC, HPTLC, UPLC and LC-MS/MS. Senior Technical Officer-II, Patent Cell Division Experience of preparing comprehensive s cientific literature/Patent documents for project using software likes SciFinder, STN, Pub Med, TOXNET, ISI Web, EPO, USPTO, JPO, WIPO etc. Technical Officer, Natural Plant Products, Instrumentation In-charge Operation and maintenance of sophisticated instruments like NMR (Bruker; 300MHz), LC-MS-MS(Q-TOF) Water’s Micromass, HPLC and Prep-HPLC (Water’s), HPTLC (Camag), GC-MS (Shimadzu). Method Development and validation by using sophisticated instruments. Research Officer, (IIIM-CSIR) 2012-2017 (IIIM-CSIR) 2007-2011 (IIIM-CSIR) 2002-2007 (IHBT-CSIR) 2000-2002 Govt. Opium & Alkaloid Works, Neemuch

1998-2000 Govt. Opium & Alkaloid Works, Neemuch Senior

Analysis of opium samples and its derivatives by using GC,HPLC, GC-MS and conventional methods. Analysis of store samples : Activated carbon, Filter aid, 1989-1992 M/S Hydroair Controls, Mumbai Chemist Waste Water Treatment analysis” of different factories in India. Analysis of Chemical and Biological Oxygen demand, Oil & Greese, Dissolved oxygen, Hardness, Acidity, Alkanity Furnace oil, Filter cloth and pH.

Scientific Assistant,

3 • Training attended at Indian Pharmacopoeia Commission in Analytical Division, IPC, Ghaziabad 18 th to 29 th Dec. 2017. • Three days training attended on “ Liquid Chromatography Mass Spectroscopy Hands-on ”, Organised by M/S Waters India, 15-17 th May 2019. MEMBERSHIP OF SCIENTIFIC SOCIETIES 1. Life member of “National Magnetic Resonance Society”. 2. Life member of “Essential Oil Organization of India”. 3. Life member of “Indian Society of Analytical Scientists”. 4. Life membership “Chromatographic Society of India”. (CSI). 5. One year member of “Society for Medicinal Plants and Natural Product Research”. 6. One year member of “International Society for Ethnopharmacology”. WORKSHOPS & TRAININGS ATTENDED • VII National Users Workshop on “Application of High Resolution NMR, Mass Spectrometry & • Electron Microscopy”, Jan. 4-6, 1995 at Central Drug Research Institute, Lucknow, India. • Training on “HPLC-Practice of Liquid Chromatography”, Jan. 6-8, 1997 at Lab India Instruments Pvt. Ltd, Pune, India (An associates of Perkin Elmer). • Workshop on "Sophisticated Instruments in Biomedical R & D Activities", March 20, 1997, Central Drug Research Institute, Lucknow, India. • Training on "High Performance Thin Layer Chromatography", in ANCHROME Enterprises (Recognised by CAMAG, Switzerland) at Bombay, August 17-18 , 1999. • Participated in the National School on “A New Dimension to NMR: From Molecules to Human Behavior” from June 15-June 25, 2004 at IHBT, CSIR. Palampur, Himachal Pradesh (India). • Training on “QTOF micro small molecules operator training course”, at Manchester (UK), 23-26 Jan. 2006. • Training on “GC-MS Techniques, Troubles-shooting and GCMS solution Training for GCMS- QP2010” , 04-06 Sep 2006 at Shimadzu (Asia Pacific) Pte Ltd, Singapore • “Workshop on Advance Workshop on Patent Strategies” , from 1 st to 4 th Feb 2010, at Human Resource Development Center (CSIR), Kamla Nehru Nagar, Ghaziabad. • Attended three days symposium on “5 th International Symposium on Drug Metabolism & Pharmacokinetics (DMPK)”, from 7 th to 10 th March 2013 at NIPER, Mohali. • 5 days NABL Assessor training, “ Laboratory Assessors Course as per ISO/IEC 17025:2005 ”, at NITTR, Chandigarh 21 to 25 th 2017 organised by NABL, Gurgaon. • Workshop Attended, “ Indo-USP workshop on Pharmacopoeial Monographs of Ayurvedic/Herbal Medicines ”, Organised by PCIM & H and USP,USA at Delhi 7 th Nov 2017.

4 Gupta, A. P. , Singh, B. and Kaul, V. K. “Simultaneous Determination of Artemisinin, Artemisinic acid and Arteanniun-B in Artemisia annua using Reverse Phase High Performance Thin Layer Chromatography”, J. of Separation Science, 28, 2288-2292, 2005 . 6. Kumar, N., Singh, B., Bhandari, P., Gupta, A. P. , Uniyal, S. K., and Kaul, V. K. “Biflavonoids from Lonicera japonica ”, Phytochemistry, 66 , 2740-2744, 2005 . 7. Swaroop, A., Gupta, A. P. and Sinha, A. K. “Simultaneous determination of Quercetin, Rutin and Coumeric Acid in Flowers of Rhododendron arboreum by HPTLC”, Chromatographia, 62 , 649-652, 2005 . 8. Singh, N., Mahmood, U., Kaul, V. K., Gupta, A. P. and Jirovetz, L. “A new alkylated benzoquinone from rhizomes of Iris kumaonensis ”, Natural Product Research , 20 , 75-78, 2006 . 7. Life member of “The Indian Science Congress Association”. 8. Three years member of The Phytochemical Society of Europe. 9. Member of Science Advisory Board (SAB). 10. Editorial Board member “International Journal of Ethnopharmacology”. 11. Editorial Board member “Journal of Management Studies”. 12. Editorial Board member, “Journal of Homeopathy & Ayurvedic Medicine”. 13. Editorial Board member, “International Journal of Herbs, Spices and Medicinal Plants”. 14. Editorial Board member, “International Journal of Pharma and bio sciences”. 15. Editorial Board member, “International Journal of Pharma And Chemical Research (IJPACR)”. 16. Editorial Board member, “Journal of New Science Biotechnology”. 17. Editorial Board member, “Austin Journal of Biotechnology & Bioengineering”. 18. Editorial Board member, “Global Journal of Biotechnology and Biomaterial Science”. 19. Editorial Board member, “Innovational: Journal of Quality Assurance and Pharma Analysis (IJQAPA)”. 20. Editorial Board member, “Global Journal of Organic and Inorganic Chemistry and Global Journal of Chemistry and Materials”, Journal of Innovations in Pharmaceuticals and Biological Sciences (JIPBS), SM Journal of Nutrition and Metabolism. 21. Guest reviewer “Food and Nutrition Sciences”. 22. Editorial Board members, “Pharmaceutical Crop”, Platinum Global Journal of Medicine and Medical Sciences (PGJMMS). 23. Reviewer “Chromatography Research International, J of Sep Science, Food Chemistry, Food and agriculture chemistry, J of AOAC, Chromatographia, International Journal of Research & Development Technology (IJRDT), Natural Product Communication, Natural Product Research, Analytical method, J of liquid chromatography and related technologies, Phytomedicine, J of chromatographic science etc. 24. Peer reviewer “Research and Reports in Biology”. PUBLICATION LIST & OTHERS: 1. Mishra, N., Gupta, A. P. , Singh, B., Kaul, V. K. and Ahuja, P. S. “Rapid Determination of Podophyllotoxin in Podophyllum hexandrum by Reverse Phase High Performance Thin Layer Chromatography”, J. of liquid chromatography and related technologies , 28, 665-679, 2005 . 2. Singh, N., Gupta, A. P. , Singh, B. and Kaul, V. K. “Quantification of Picroside-I and Picroside-II in Picrorhiza kurroa by High performance thin layer chromatography”, J. of liquid Chromatography and related technologies, 28, 1679-1691, 2005 . 3. Mishra, N., Acharya, R., Gupta, A. P. , Singh, B., Kaul, V. K. and Ahuja, P. S. “A simple Micro analytical Technique for the Determination of Podopyllotoxin hexandrum Roots by Quantitative RP- HPLC and RP-HPTLC”, Current Science, 88, 1372-1373, 2005 . 4. Singh, N. P., Gupta, A. P. , Sinha, A. K. and Ahuja, P. S. “High Performance Thin Layer Chromatography method for quantitative Determination of four major Anthraquinone derivatives in Rheum emodi ”, J. of Chromatography A, 1077,202-206, 2005 . 5. Bhandari, P.,

9. Singh N., Gupta, A. P. , Singh, B. and Kaul, V. K. “Quantification of Valerinic Acid in Valeriana jatamansi and Valeriana officinalis by HPTLC”, Chromatographia , 63 , 209-213, 2006. 10. Kumar, N., Singh, B., Gupta, A. P. , and Kaul, V. K. “Lonijaposides, novel cerebrosides from Lonicera japonica ”, Tetrahedron, 62 , 4317-4322, 2006 . 11. Pathania, V., Gupta, A. P. and Singh, B. “Improved HPTLC Method for Determination of Curcuminoids from Curcuma longa”, J. of liquid chromatography and related technologies , 29 , 877- 885, 2006 . 12. Bhandari, P., Gupta, A. P. , Singh, B. and Kaul, V. K. “HPTLC Determination of Swertiamarin and Amarogentin in Swertia species from the Western Himalayas”, J. of Planar Chromatography , 19 , 212-215, 2006 . 13. Bhandari, P., Kumar, N., Gupta, A. P. , Singh, B. and Kaul, V. K., “Micro-LC Determination of Swertiamarin in Swertia species and Bacoside-A in Bacopa monnieri by HPLC”, Chromatographia , 64, 599-602, 2006 . 14. N. Kumar, P. Bhadari, B. Singh, A. P. Gupta and V. K. Kaul, “Steroidal alkaloids from Holarrhena antidysenterica ”, Chem. Pharm. Bull . , 55(6), 912-914, 2007 . 15. Chawla, R., Arora, R., Singh, S., Sagar, R. K., Sharma, R. K., Kumar, R., Sharma, A., Gupta, M. L., Singh, S., Prasad, J., Khan, H. A., Swaroop, A., Sinha, A. K., Gupta, A. P. , Tripathi, R. P., Ahuja, P. S., “Radio- protective and antioxidant activity of fractionated extracts of berries of Hippophae rhammnoides ”, J. of Medicinal Food , 10(1), 101-109, 2007 . 16. Sharma, U. K., Sharma, N., Gupta, A. P ., Kumar, V. and Sinha, A. K., “RP-HPTLC determination and validation of vanillin and related phenolic compounds in accelerated solvent extract of Vanilla planifolia : A comparison with microwave, ultrasound and soxlet extraction”, J. of Separation Science , 30, 3174-3180, 2007 . 17. P. Bhandari, N. Kumar, A. P. Gupta , B. Singh, V. K. Kaul, “A rapid RP-HPTLC densitometry method for simultaneous determination of major flavonoids in important medicinal plants”, J. of Separation Science, 30(13), 2092-2096 , 2007 . 18. N. Kumar, P. Bhadari, B. Singh, A. P. Gupta and V. K. Kaul, “Reversed phase-HPLC for rapid determination of polyphenols in flowers of rose species”, J. of Separation Science , 31, issue-2, 262-267, 2008 . 19. V. Sharma, A. P. Gupta , P. Bhandari, R. C. Gupta and B. Singh, “A validated HPTLC method for the quantification of withaferin-A and withanolide-A in different plant parts of two morphotypes of withania somnifera ”, Chromatograhia , 66, 801-804, 2007 . 20. V. Jaitak, A. P. Gupta, V. K. Kaul and P. S. Ahuja, “Validated high performance thin layer chromatography method for steviol glycosides in S. rebaoudiona ”, J. of Pharmaceutical & Biomedical Research , 47, issue-4-5, 790-794, 2008 . 21. N. Kumar, P. Bhadari, B. Singh, A. P. Gupta and V. K. Kaul, “Reversed phase-HPLC for rapid determination of polyphenols in flowers of rose species”, J. of Separation Science , 31, 262-267, 2008 . 22. Pati P. K., Sharma M., Sharma A., Salar R. K., A. P. Gupta and B. Singh “Studies on leaf spot disease of Withania somnifera and its impact on secondary metabolites.” Indian J of Microbiology , 48, 432-437, 2008 . 23. P. Bhandari, N. Kumar, B. Singh, A. P. Gupta , V. K. Kaul and P. S. Ahuja “Stability-indicating LC-PDA method for determination of picrosides in Hepato-protective Indian herbal preparations of Picrorhiza kurroa ”, Chromatographia , 69, 221-227, 2009 . 24. N. Sharma, U. Sharma, A. P. Gupta , Devla, A. K. Sinha, B. Lal and P. S. Ahuja, “Simultaneous densitometric determination of shikonin, acetylshikonin and β -acetoxyisovalerylshikonin in ultrasonic-assisted extracts of four Arnebia species using reversed-phase thin layer chromatography”, J. of Separation Science , 32, 3239-3245, 2009 . 25. Upendra K Sharma, Nandini Sharma, Arun K Sinha, Neeraj Kumar, A. P. Gupta , “Ultrafast UPLC- ESI-MS and HPLC with Monolithic Column for determination of Principal flavor Compounds in vanilla pods” Journal of Separation Sciences , 2009, 32, 3425-3431.

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26. N. Sharma, U. K. Sharma, A. P. Gupta , and A. K. Sinha, “Simultaneous determination of epicatechin, syringic acid, quercetin-3-O-galactoside and quercitrin in the leaves of Rhodendron species by using a validated HPTLC method, J. of Food Composition and Analysis , 23, 214-219, 2010. 27. I. Khan, P. Pandotra, A. P. Gupta, R. Sharma, B. D Gupta, J. K. Dhar, G. Ram, Y. S. Bedi, S. Gupta, “RP- Thin layer chromatographic method for the quantification of three gingerol homolog’s of ultrasonic-assisted fresh rhizome extracts of Zingiber officinale Collected from North Western Himalayas”, J. Separation Science , 33, 558-563, 2010 . 28. S. Gupta, P. Pandotra, A.P. Gupta , J. K. Dhar, G. Sharma, G. Ram, M. K. Husain and Y. S. Bedi, “Volatile (As and Hg) and non-volatile (Pb and Cd) toxic heavy metals analysis in rhizome of Zingiber officinale Collected from different locations of North Western Himalayas by Atomic absorption spectroscopy”, Food and Chemical Toxicology , 48, 2966-2971, 2010 . 29. S. Gupta, P. Pandotra, G. Ram, Rajneesh Anand, A. P. Gupta, Y. S. Bedi and G. R. Mallavarapu, “ Characterization of monoterpenoids-rich essential oil from the rhizome of Zingiber officinale Collected from different locations of North Western Himalayas by capillary gas chromatography-ion trap mass spectrometry, Natural Product Communications , 6(1), 93-96, 2011. 30. Suphla Gupta, Rajni Sharma, Pankaj Pandrotra and Ajai Prakash Gupta, “Separation and System Suitability Analysis for Monolithic Reversed Phase LC of Gingerols and Shogaol”, Separation science, 3(2), 5-8, 2011. 31. Suphla Gupta, Rajni Sharma, Pankaj Pandotra, A. P. Gupta , “Chromolithic Method Development, Validation and System Suitability Analysis of Ultra-Sound Assisted Extracted Anti-Cancer Glycyrrhizic Acid and Glycyrrhetinic Acid from Glycyrrhiza Glabra” , Natural Product Communications , 7(8), 991-994, 2012 . 32. R. Mudududdla, S. K. Jain, J. B. Bharate, A. P. Gupta , B. Singh, R. A. Vishwakarma and S. B. Bharate, “ortho-Amidoalkylation of Phenols via Tandem One-Pot Approach Involving Oxazine Intermediate”, J. Org. Chem. , 77, 8821 − 8827, 2012 . 33. B. A. Wani, D Ramamoorthy, M. A. Rather, N. Arumugam, A. Hamid, S. A. Ganie, B. A. Ganai, R. Anand, A. P. Gupta , “Induction of apoptosis in human pancreatic MiaPaCa-2 cells through the loss of mitochondrial membrane potential (ΔΨm) by Gentiana kurroo root extract”, Phytomedicine: International journal of phytotherapy and phytopharmacology , 20, 723-733. 2013. 34. P. Pandotra. A. P. Gupta , Gandhiram , Mohd K. Husian, S. Gupta , “Genetic and Chemo-divergence in Eighteen Core Collection of Zingiber officinale from North-West Himalayas”, Scientia Horticulturae , 160, 283-291, 2013 . 35. S. Gupta, P. Pandotra , A. P. Gupta , M. K. Verma, A. Ahuja , R. A. Vishwakarma, “Direct rhizogenesis, in vitro stolon proliferation and high throughput regeneration of plantlets in Glycyrrhiza glabra ”, Acta Physiologeae Plantarum, Doi: 10.1007/s11738-013-1302-1, 2013 . 36. P. Pandotra, Mohd K. Husain, Gandhiram, S. Gupta, A. P. Gupta , “Evaluation of Genetic Diversity and Chemical Profile of Ginger Cultivar in North-western Himalayas”, Biochemical Systematics and Ecology , 48, 281–287, 2013 . 37. P. Pandotra, Mohd K. Husain, Gandhiram, S. Gupta, A. P. Gupta , “Retrotransposon based genetic status of North-West Himalayan Zingiber officinale revealed high heterogeneity”, Journal of Plant Biochemistry and Biotechnology , DOI 10.1007/s13562-013-0196-8, 2013 . 38. J. B. Bharate, R. A. Vishwakarma, S. B. Bharate, T. B. Thite, M. Kushwaha, A. P. Gupta , “Quantification and Validation of Two Isomeric Anticancer Compounds, Garcinol and Isogarcinol, in Ultrasound-Assisted Extracts of Garcinia indica Fruits Using High-Performance Thin-Layer Chromatography”, J. of Planar Chromatography , 26, 480-485, 2013 . 39. S. Gupta, R. Sharma, P. Pandotra, P. Datt, A. P. Gupta , “Chromolithic RPLC-PDA method development, validation and quantification of gingerols and shogaol by ultra-sound assisted extracted rhizome of Zingiber officinale”, International Journal of Green Pharmacy , 7, 189- 195, 2013 . 40. P. Pandotra, B. Viz, Gandhi Ram, A. P. Gupta , and S. Gupta, “Multi-elemental profiling and

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