FOL-02

METHOD FOR THE ESTIMATION OF TOTAL PHENOLIC CONTENT USING THE FOLIN- C ASSAY

Results of validation: Following test were conducted to verify the performance of the method as per the requirement of AOAC SMPR 2015.009.

10.0

10.1. Analytical Range: Analytical range was demonstrated on reference standard. Different concentrations ranging from 5-100ppm were prepared and measure the absorbance.

Sr.no.

Concentration (ppm)

Absorbance

1

5

0.101

2

25

0.325

3

50

0.651

4

100

1.273

Calibration Curve of Gallic acid

1.4

1.2

1

y = 0.012x + 0.028 R² = 0.999

0.8

0.6

Abosrabance

0.4 Absorbance

0.2

0

0

50

100

150

Concentartion in ppm

Conclusion: The Co-relation coefficient (R 2 ) of 0.999 confirms that the concentrations ranges are linear and follows the Beers lambert’s laws.

10.2. Limit of Quantification (LOQ): Based on Linearity study a concentration of 5 ppm is found to be the LOQ. Six different preparation of 5ppm were analyzed and the relative standard deviation (RSD) is within 10%. Confirming LOQ to be 5ppm 10.3. Recovery: Recovery study was conducting by adding known amount (80 to 120% of working range) of standard in both the raw material & extract. The samples were analyzed using the methodology mentioned above (7.4). The recovery is within 80 to 110%.

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