FOL-02

Estimetion of Total Phenolic content using the Folin-C assay

Validation Data Of Polyphenol content by Folin Ciocalteu's reagent in Salacia

Analytical Range:

1)

Gallic acid Concentartion in ppm Absorbance

Sr.No.

1 2 3 4

5

0.101 0.325 0.651 1.273

25 50

100

Calibration curve of Gallic acid

0 0.2 0.4 0.6 0.8 1 1.2 1.4

1.273

y = 0.0124x + 0.0285 R² = 0.9996

0.651

0.325

Absorabance

0.101

0

20

40

60

80

100

120

Concentartion in ppm

Limit of Quantification:

2)

Weight Of Standard (mg)

Absorbance of 5ppm concentration

50.6 50.2 51.2 49.5 50.4 50.2

0.101 0.109 0.118 0.101 0.102 0.098

Average

50.35

0.10 0.01 7.07

Standard Deviation

0.56 1.11

% RSD

Recovery For Raw material (B.No-OA150152) Range (%)

3)

Theoratical Value

Practical Value

% Accuracy

80

1.96 2.46 2.95

2.00 2.44 3.08

102.04

100 120

99.19

104.41

For Extract (B.No-SREE-00-080202002) Range (%)

Theoratical Value

Practical Value

% Accuracy

80

17.60 22.26 25.90

17.23 23.45 26.22

97.90

100 120

105.35 101.24

Repeatability

4)

Gallic acid

II Dilution

Weight Dilution

50.6

0.5

50 99

10

Purity

Absorbance

0.651

II-Dilution

Sample Details

Sample wt

Dilution

Absorbance

Result (%)

OA150152-T1 OA150152-T2 OA150152-T3

2076.70 2011.20 2005.80

50 50 50

2 2 2

25 25 25

1.063 1.033 0.996

2.46 2.47 2.39 2.44 0.05 1.85

Average

SD

%RSD