Fluoride Working Group Meeting Book (December 14, 2017)

Ion Chromatography Method (Fluo-02) ThermoFisher ^ĐŝĞŶƚŝĨŝĐ͘ ŶĂůLJƐŝƐ of fluoride in Infant Formula and Adult NƵƚƌŝƚŝŽŶĂůƐ ďLJ ŝŽŶ ĐŚƌŽŵĂƚŽŐƌĂƉŚLJ ĂĨƚĞƌ ƵůƚƌĂĐĞŶƚƌŝĨƵŐĂƚŝŽŶ • 'ŽŽĚ ůŝŶĞĂƌŝƚLJ ;х Ϭ͘ϵϵϱͿ ĂŶĚ ĞƐƚŝŵĂƚĞĚ >KY ;у ϱђŐͬϭϬϬŐͿ • Good Z^ ƌ ;ф ϱйͿ ͕ Z^ Z ;фϭϬйͿ ĂŶĚ ƌĞĐŽǀĞƌLJ ;ϴϭй-ϭϬϱйͿ ĨŽƌ ϭϮ ^W/& E ƉƌŽĚƵĐƚƐ • ^ƉĞĐŝĨŝĐ ĞƋƵŝƉŵĞŶƚ ĂŶĚ / ĐŽŶƐƵŵĂďůĞƐ ;ĐŽůƵŵŶ͕ ĐĂƌƚƌŝĚŐĞƐ͕ ďƵĨĨĞƌͿ ŶĞĞĚĞĚ ͬ Chloride ĂŶĂůLJƐĞĚ ƐŝŵƵůƚĂŶĞŽƵƐůLJ Conclusion: equipment dependent / method to be improved for low F level (e.g. removal of organic acids͕ column and buffer used͕ sample volume injected) for a better chromatogram interpretation ͬ ŽŵƉůĞƚĞ ^>s Θ additional ^>s chloride could be a plus using improved method

September 2016

9

Reference 10 is listed in slide 16

Microwave acid digestion and F-ISE 10

• Alternative to tedious & time consuming alkali fusion or microdiffusion • >KY blank͗ ϱ ђŐͬ> ͬ >KYƐĂŵƉůĞ у ϮϬ ђŐͬϭϬϬŐ ĚƌLJ matter (1g with &сϮ d/^ ϮϬйͿ ͬ 20 food samples (seafood͕ vegetables͕ ĨƌƵŝƚƐ͗ Ϭ͘Ϭϴ – ϭ͘ϴ ŵŐͬϭϬϬŐ ĚƌLJ matter) • Not validated for infant formula: (estimated >KY ͗ф ϮϬ ђŐͬϭϬϬŐ Zd&Ϳ Conclusion: ĨĞĂƐŝďŝůŝƚLJ to test for Infant Formula with some modifications (e.g. diluted HNO 3 / 2g IF reconstituted / F-/^ ĐĂůŝďƌĂƚŝŽŶ ƚLJƉĞͿ 10 September 2016