ISPAM Advisory Panel-Food Allergen

TLA Panel Allergens: priorities and challenges for food allergen detection 㻶㼡㼚㼑㻌㻝㻡㻘㻌㻞㻜㻝㻢

MPR for gluten: CEN activities (III) - Structure follows CEN requirements but also contains important AOAC SMPR elements - Title : Minimum Performance Requirements for quantitative gluten measurement by ELISA - Scope : …specifies minimum requirements on information and performance for immunological methods that quantify intact or fragmented gluten from wheat, rye, and barley in raw and processed foodstuffs. Information on applicability, antibody, construction of the test system, calibration material, curve fitting algorithm, and reporting of results shall be given by the method provider. Performance characteristics that shall be validated for each method are specificity, selectivity, limit of detection, limit of quantification, cross reactivity, interfering substances, recovery, precision, accuracy, trueness, and robustness.

TLA Panel Allergens: priorities and challenges for food allergen detection 㻶㼡㼚㼑㻌㻝㻡㻘㻌㻞㻜㻝㻢

MPR for gluten: CEN activities (IV) - Normative References - Terms and Definitions: gluten, prolamin, exception for oats - General information on the test system - Performance criteria (how to determine…..) - Overview on Minimum Performance Requirements - Table 1: Intact gluten - Table 2: fragmented gluten Parameter Analytical Range [mg/kg]

Gluten

10 to 40

LOQ [mg/kg] LOD [mg/kg] Recovery [%]

10

5

70 to 130

RSD r [%] RSD R [%] Specificity

15

30

Gluten from wheat, rye, and barley