ISPAM Advisory Panel-Food Allergen

6/14/2016

Contribution from I. Taverniers (ILVO) & N. Gillard (CER)

Belgian NRL-Allergens (2 labs + Federal Food Safety Agency) Validation study on peanut detection - Conclusions

• ELISA and qPCR useful for peanut detection and quantification • Quantities obtained by qPCR in rice might be slightly more accurate (more true compared to the expected/theoretical values) than quantities obtained by ELISA • Kit to kit variation • Matrix to matrix variation:  Baked cookies, cumin powder less applicable matrices in this study (influence of processing )  Effect of mixing flour to other cookies ingredients and the effect of high-temperature baking clearly observed, in terms of less detection and quantification in both ELISA and qPCR  This effect was even stronger for qPCR compared to ELISA • Variation between the two labs who used exactly the same test materials for exactly the same set of 11 peanut test kits

Factors influencing ELISA measurements

Antibodies



Monoclonal or polyclonal

 Target of the antibody: single/several proteins, fractionated,

modified, synthesized

 Material used for immunization

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