ISPAM Advisory Panel-Food Allergen

6/14/2016

What are we looking for?

Identify the target:

Raw protein or processed commodity?

 Choose antibodies accordingly  Similar calibrators  SMPR’s can harmonize allergen methods, but will be most effective if allergen reference standards identified

Factors influencing ELISA measurements

Calibrators

 Match the antibodies of the kit

 Not the same as sample material

Extraction

 What is extracted (efficiency)?

 Antibody can only detect what has previously been detected

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