ISPAM Advisory Panel-Food Allergen

6/14/2016

Matrix Choices: Allergen Material

• Incurred •

Better option – allergen processed along with food

matrix

• True evaluation of extraction efficiency

• Difficult to design/predict allergen levels precisely

• Challenge for method developer

• Some incurred RM available for purchase

No CRM

•

• Reasonable goal could be one incurred RM per

allergen for validation of trueness

Matrix Choices: Allergen Material

• Spike •

Necessary when validating more than a

couple matrices

• Necessary for certain matrices which cannot

be produced in lab

• Generally recover close to 100% of spike

• Useful for measuring precision, interference

• Allergen extract vs whole allergen

Homogeneity

•

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