ISPAM Advisory Panel-Food Allergen

6/14/2016

Factors influencing ELISA comparability

Cross reactivity  Positive response to a sample without target allergen

 Test products genetically similar to target allergen

 Different response than in study possible:

- raw vs. processed product

- variations within globally used cultivars

Factors influencing ELISA comparability

Cross reactivity  Choose appropriate cross reactivity

panel based on: • Genetic closeness • Tree of life closeness • Search for conserved domains  Panel should not only be exclusive, but also inclusive: • Bovine/sheep/goat milk reactivity • Gliadin/hordein/secalin reactivity  Consider cross reactor panel choices

from international perspective

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