ISPAM Advisory Panel-Food Allergen

6/14/2016

Factors influencing ELISA comparability

Immunological test system  Environmental influences

 Experimental conditions

 Stability affected by transport & storage conditions

 Robustness vs ruggedness

Summary

 Food allergens are extremely diverse  Significant variability of results between immunoassay methods, labs, matrices  No certified reference standard  no common reference point for ELISA comparison  Many matrix factors influence accuracy – take into account when choosing matrices  Consider both inclusivity and exclusivity when testing cross reactors  Consider both robustness and ruggedness

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