ISPAM Advisory Panel-Food Allergen

Allergen Regulatory Testing

• Two Different Test Kits • Encompass antibody differences and extraction differences • Analyze in triplicate (minimum) • Multiple Controls

• False Negative • False Positive • Dynamic Range • Determine and identify analyst error • Additional confirmation testing may be performed • Multi-allergen antibody detection (profiles) • DNA (PCR and/or “barcoding”) • Mass Spectrometry • Western blots

• Appropriate Standards for Hydrolyzed and Fermented Foods • Appropriate Standards for Processed Foods • Multi-allergen antibody detection (profiles/cross reactivity) • Measure of Cross Reactivity • Clear protocols: • Fortification/Recovery • Cross Reactivity Technical Challenges