ISPAM Advisory Panel-Food Allergen

Is food allergen analysis flawed ? (Cont’d) • Risk assessment and risk management of food allergens depend on the ability to detect and accurately quantify them reproducibly. • All current analytical methods exhibit deficiencies that jeopardize accurate results and risk false positives and false negatives. • ELISA is widely used for an extensive period of time, and evidence of deficiencies are published. • PCR and LC-MS/MS are newer technologies, similar deficiencies are not studied but are expected to be similar. o Better proteomic and genomic databases / bioinformatics help • Uncertainties remain: 9 Clinical vs Bioinformatics 9 Reference material vs calibrants 9 Allergen structural changes by food processing

9 Impact of food matrix 9 Extraction efficiency 9 Quantification depends on markers chosen 9 Diversity of foods and commodities in global market

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Complexity of allergen sampling plan

CONFIDENTIAL Proprietary information of Nestlé S. A., Vevey, Switzerland This document should not be reproduced or disclosed without prior authorisation