ISPAM Meeting Book 3-15-16

• Official Methods of Analysis of AOAC INTERNATIONAL (2012) 19th Ed., AOAC INTERNATIONAL, Gaithersburg, MD, USA, APPENDIX I; also on-line at http://www.eoma.aoac.o

Identification of Venezuelan Equine Encephalitis Virus (VEEV)

Parameter

Minimum Performance Requirement

AMIL

5000 genome copies / mL

POI at AMIL within sample collection buffer POI at AMIL in an aerosol environmental matrix System False-Negative Rate using spiked aerosol environmental matrix System False-Positive Rate using aerosol environmental matrix

≥ 0.95

≥ 0.95

≤ 5%

≤ 5%

All inclusivity strains (Table II) must be correctly identified at 2x the AMIL † All exclusivity strains (Table III and Annex IV; part 2) must test negative at 10x the AMIL †

Inclusivity panel purified DNA

Exclusivity panel purified DNA