ISPAM Working Group Chair Presentation Book

Thin Layer Chromatography (TLC) d l d f d • Screening an pre iminary i enti ication imite to  known analogs with reference standards • Cheap, fast but not very sensitive • HPTLC  can improve sensitivity (densitometric analysis of spots can be used for quantitation) Examples: ‐ Cai et al., J. Liquid Chromatogr. Relat. Technol. 33 (2010) 1287–1306  (8 compounds) ‐ Abourashed et al., J. Planar Chromatogr. Mod. TLC 18 (2005) 372–376 (sildenafil) ‐ Reddy et al., J. Planar Chromatogr. Mod. TLC 19 (2006) 427–431 (sildenafil) l