ISPAM Working Group Chair Presentation Book

Mass Spectrometry (MS) Di MS i h h • rect   w t out c romatograp c separat on  (Ambient MS or Flow‐Injection MS) • GC‐MS – limited use for identification • LC‐MS – prevalent technique for PDE5 inhibitors Benefits of MS: • Sensitivity • Selectivity – tandem MS (MS/MS) and accurate mass‐ high resolution (AM/HR) MS and MS/MS • Structure‐based identification/confirmation • Can be combined with UV or fluorescence detection • Suitable for both targeted and non‐targeted analysis hi i