ISPAM Working Group Chair Presentation Book

Challenges • ELISA kits oftentimes are used by technicians who are not experienced  at LC/MS/MS.

• Allergens are present in many matrices.

• Method development to determine allergen proteins is time  consuming.

• Most food testing labs are used to using LC/MS/MS for small molecule  contaminant testing.

• Sample preparation for allergens (protein digestion) is significantly  different from current sample prep methods (LLE, QuEChERS, SPE, etc)