ISPAM Working Group Chair Presentation Book

Existing LC/MS/MS Methods • Revision of the sample 

Extract proteins from food sample (1 hour in buffer)

preparation utilizing  automated online SPE

• Total sample preparation  time down to 4.5 hours • Results achieved through  modification: • Prep‐time reduced by 4x • Sensitivity improved by 2‐4x • LC‐MS/MS run time reduced by nearly 2x • Addition of more allergens  (milk, egg, peanut, treenuts,  l t t d) g u en, sesame, mus ar

Centrifuge and collect extract (30 min)

Reduce, alkylate, and di t ith t i ( 2 5 ges w ryps n . hours )

Filter digested extract (15 min)

Perform automated online SPE clean-up ( 0 hours hands-on time )

LC-MS/MS analysis