ISPAM Working Group Chair Presentation Book

Fitness for Purpose

• Benefits of LC‐MS/MS:

XIC of +MRM (71 pairs): Exp 1, 929.986/1116.601 Da ID: [e]ELINSWVESQTNGIIR from Sample 1 (E100 mrm epi 4 allergen 161208 1) of E100 mrm e...

Max. 7100.0 cps.

1.5e4 2.0e4 2.5e4 2.8e4

Egg peptides

– Improved throughput through multiplexing – Multiple allergens in  one injection – Multiple fragments for  each allergen detected More confidence for – fewer false positive or  false negative results

nsity, cps 1.0e4

12.69

Inte

5000.0

0.0

1.0

2.0

3.0

4.0

5.0

6.0

7.0

8.0

9.0

10.0 11.0 12.0 13.0 14.0 15.0 16.0

Time, min

XIC of +MRM (71 pairs): Exp 1, 688.830/833.400 Da ID: [p]DLAFPGSGEQVEK from Sample 1 (P100 mrm epi 4 allergen 161208 1) of P100 mrm epi 4 ...

Max. 9900.0 cps.

2 0e5 2.5e5 3.0e5

Peanut peptides

ps 0.0 5.0e4 1.0e5 1.5e5 . Intensity, c

9.67

7.27

1.0

2.0

3.0

4.0

5.0

6.0

7.0

8.0

9.0

10.0 11.0 12.0 13.0 14.0 15.0 16.0

Time, min

XIC of +MRM (71 pairs): Exp 1, 684.350/1183.570 Da ID: [m]ALNEINQFYQK from Sample 1 (M100 mrm epi 4 allergen 161208 1) of M100 mrm epi 4 all...

Max. 7.7e4 cps.

8.0e5 8.8e5

Milk peptides

0.0 1.0e5 2.0e5 3.0e5 4.0e5 5.0e5 6.0e5 7.0e5 Intensity, cps

10.08

1.0

2.0

3.0

4.0

5.0

6.0

7.0

8.0

9.0

10.0 11.0 12.0 13.0 14.0 15.0 16.0

Time, min

Multi‐allergen analysis