July 2019 Flavanols ERP - Review Book

Method Feedback for First Action AOAC method being considered for Final Action: In the use of AOAC Official Method 2012.24 Flavanol and Procyanidin ( by Degree of Polymerization 1-10) of Chocolate, Cocoa Liquor, Powder(s) and Cocoa Flavanol Extracts, a contract lab that did testing for our company used two different analytical columns for HPLC separation of PAC 1-10. They applied the same response factors to the peaks as outlined in the method to both separations without any adjustment. Column indicated in the published method is Develosil diol 100 Å 5 μm particle size, 250 × 4.6 mm id (Phenomenex, Torrance, CA; Cat. No. DI11546250W). Method indicates that column specified be used or its equivalent. The other column used by the lab was Luna HILIC 200 Å 5 μm particle size, 250 × 4.6 mm id (Phenomenex, Torrance, CA; Cat. No. 00G-4450-E0). Both the lab that did the testing for us and I agree that in some cases there is a significant bias between the quantitation of the higher polymer components, especially pentamer through decamer that doubles the total procyanidins reported if the Luna HILIC column is used as outlined in the method. There needs to be more specific requirements provided for defining equivalence of the column. Diana Kavolis, Staff Scientist, The Hershey Company - 717-534-6369. Dkavolis@hersheys.com