KRA-01

FDA/ORA/ORS

LIB #4578 21 of 25

Figure 17: a) Positive Control - Total Ion Chromatogram (TIC)

b) Positive Control - Reconstructed Ion Chromatogram (RIC)

Kraton_27Dec12_A14 - TIC - SM: 5 RT: 5.00 - 14.99

NL: 2.12E6

RT: 11.51

10 15 20 25 30 35 40 45 50 55 60 65 70 75 80 85 90 95 100 Relative Intensity

a

RT: 12.26

RT: 12.79

RT: 13.25

0 5

13.55 14.11 14.70

5.31

6.09

6.98

7.66

8.42

9.07

9.56

RT: 10.24

11.03

6

7

8

9

10

11

12

13

14

Time (min)

RT: 9.86 - 14.99

RT: 10.50 - 15.00

RT: 11.55

NL: 1.78E6 TIC F: + c ESI SRM ms2 399.200 [159.099-159.101, 174.099-174.101, 238.199-238.201] MS ICIS kraton_28dec12_a15

RT: 11.55

NL: 7.99E5 m/z= 173.90-174.10 F: + c ESI SRM ms2 399.200 [159.099-159.101, 174.099-174.101, 238.199-238.201] MS ICIS kraton_28dec12_a15

100

10 20 30 40 50 60 70 80 90 100

95

b

90

SRM 399.1 -> 174 Positive Control Mitragynine Rt=11.53

85

80

RT: 12.29

75

Total Ion Chromatogram (TIC) Postive Control Mitragynine Rt=11.53

RT: 12.80

70

10 20 30 40 50 60 70 80 90 100 Relative Abundance 0

NL: 5.09E5 m/z= 159.00-159.20 F: + c ESI SRM ms2 399.200 [159.099-159.101, 174.099-174.101, 238.199-238.201] MS ICIS kraton_28dec12_a15

RT: 11.55

65

60

SRM 399.1 -> 159.1 Positive Control Mitragynine Rt=11.53

55

50

RT: 12.29

45

Relative Abundance

40

RT: 12.82

35

0 10 20 30 40 50 60 70 80 90 100 0

RT: 12.29

RT: 11.53

NL: 4.78E5 m/z= 238.00-238.20 F: + c ESI SRM ms2 399.200 [159.099-159.101, 174.099-174.101, 238.199-238.201] MS ICIS kraton_28dec12_a15

30

25

SRM 399.1 -> 238.1 Positive Control Mitragynine Rt=11.53

20

15

RT: 12.27

10

RT: 12.82

5

RT: 12.82

RT: 13.56

RT: 14.64

0

10

11

12

13

14

11

12

13

14

15

Time (min)

Time (min)