KRA-01

FDA/ORA/ORS

LIB #4578 24 of 25

Table 6:

Comparison of UPLC/PDA and LC-MS/MS

Sample

Product Type

UPLC/PDA % (wt/wt)

LC-MS/MS % Difference

% (wt/wt)

Positive Control

1.025 0.985 0.540 0.708 3.540 1.015 1.440 0.681 1.412

0.973 0.919 0.536 0.699 3.100 0.859 1.215 0.620 1.064

5.07 6.67 0.74 1.27 12.4 15.4 15.6 8.96 24.6

Positive Control Duplicate

1 2 4 5 6 7 8

Powder

Leaf Powder Dry Incense

Powder of Natural Dye Mitragyba Javanica

Kulit Kaya

Armatopic Herb

Results of Samples Tested From 2012 to 2014, samples were submitted to the Denver Laboratory ranged from small packets of drinks, capsules, tea leaves, powdered leaves and spent leaves from a manufacturing processing facility. Denver analyzed over 30 samples and Table 7 is a summary of the results.

Table 7 :

Results of Samples Tested

Sample Number Sample

Confirmation GC/MS

Quantitation UPLC

1 2 3 4 5 6 7 8 9

Powder

Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y

0.54% 0.71% 0.52% 3.54% 1.02% 1.44% 0.68% 1.41%

Leaf Powder

Powder

Dry Incense

Powder of Natural Dye Mitragyba Javanica

Kulit Kaya

Armatopic Herb

Liquid Shot Material Liquid Shot Material Liquid Shot Material Liquid Shot Material

241 µg/mL 241 µg/mL 237 µg/mL 223 µg/mL 287 µg/mL 213 µg/mL 201 µg/mL 200 µg/mL 200 µg/mL 189 µg/mL 190 µg/mL

10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

Kratomite Kratomite Kratomite Kratomite Kratomite Kratomite Kratomite

light green powder

1.54%

Kratom

308 ug/mL

Dietary Supplement

1.64% 1.68% 1.60% 1.67%

Dietary Supplement , capsule Dietary Supplement , capsule Dietary Supplement , capsule