Kombucha ERP

Does the method perform according to the method"s applicability as written? If No, please explain why the method did not perform according to the method"s applicability as written.

The method performs accirding to the applicability as written in SMPR 2016.001 "Determination of low levels of ethanol as expressed as %ABV in Kombucha"

2. SAFETY CONCERNS

Are there any safety concerns identified while using or regarding use of the method? If Yes, please identify the safety concerns regarding the use of this method.

There is no safety concerns as such. The general safety precautions are addressed in the method.

3. STATISTICAL REVIEW

Is the documentation for the statistical review prior to AOAC First Action Official Methods status available and completed?

The statistical review has been completed prior to AOAC First Action Official Methods status.

4. REFERENCE MATERIALS

Are there available reference materials? Were there any concerns identified while using or regarding use of the method?

Two Certified Reference Materials were used; one at 0.1 %ABV and another at 2.5 %ABV with accuracy of 98.2% and 101.4% respectively. Ther are no concerns identified regarding the use of reference materials in this study.

5. SINGLE LABORATORY VALIDATION

Is there data demonstrating linearity, accuracy, repeatability, LOD/LOQ? If No, please explain.

SLV data were available for review. The data demonstrated linearity, accuracy, repeatrability and LOD/LOQ of the of the method for AOAC First Action Status.

6. REPRODUCIBILITY/UNCERTAINTY AND PROBABILITY OF DETECTION

Does the available documentation demonstrate Reproducibility/Uncertainty and Probability of Detection? If yes, please specify the information provided to support the reproducibility of this method as written.

The method reproducibility was demonstrated by collaborative study. The study included six Kombucha products analysed by four laboratories (ther were five collaborators, the data from one laboratory is excluded as they could not complete the testing within specified time). The %RSDR of two products were more than 6% and thereby not meeting the SMPR requirements. The ethanol levels of these samples were 0.07% which is below the analytical range specfied in the AOAC SMPR. However the analytical range of the methood is 0.04 to 4.22% ABV. The HorRat values of the precision were 0.5 to 2.

7. FINAL ACTION REQUIREMENTS