Kombucha ERP

Kombucha Method Re-submission Review Form

Submission Date

2018-09-25 11:00:39

Name

Armen Mirzoian

Email

armen.mirzoian@ttb.gov

Organization

TTB

Has the resubmitted method met the requirements outlined on September 24, 2017? Please provide justification for your answer. If specific requirements were made in the September 24 meeting, please explain how those requirements have been met or not met.

No

KOM-04 Ethanol in Kombucha by GC-FID

No validation data submitted. Therefore, I cannot evalute and recommend thhis method.

Here are comments on the availble written part.

C. REAGENTS • Why other SRM BCR 651 is not mentioned here? However, it’s mentioned somewhere in the text. D. Preparation of Standards and Solutions a. Ethanol standards and stock solutions o If the stock solution is prepared the way it’s described, the final concentration will be ~10% abv and not 1% abv as reported. b. Ethanol calibration standard solution. o Dilution of 1% abv stock solution will result in concentrations 10 times lower than reported. Dilution of 10% abv solution will result in correct concentrations. D. Sample preparation • As described in part 4 dilution of the internal standard will results in 0.01% 2- propanol is solution. This is much lower than measured ethanol concentration. Concentration of the internal standard should be comparable to the concentration of the analyte.