Kombucha ERP

Kombucha Method Re-submission Review Form

Submission Date

2018-09-26 10:39:57

Name

Blake Ebersole

Email

blake@npscientific.com

Organization

NaturPro Scientific

Has the resubmitted method met the requirements outlined on September 24, 2017? Please provide justification for your answer. If specific requirements were made in the September 24 meeting, please explain how those requirements have been met or not met.

Yes

A. Measurement of precision and accuracy in the kombucha matrix was performed:

1. 4 commercial samples tested in triplicate on 2 different days and analysts was performed.

--The repeatability ranged from 0.65 to 1.27% RSD at mean concentrations ranging from 0.78 to 1.52% ABV.

2. 4 commercial samples were spiked with 3 concentrations of ethanol certified reference material in the range of 0.253 to 0.507.

- The percent recovery of these samples ranged from 98.33 to 101.92

3. 2 commercial fruit juice samples were spiked with 3 concentrations of ethanol certified reference material in the range of 0.253 to 0.507.

- The percent recovery of these samples ranged from 95.7 to 101.2

B. Precision and accuracy using ethanol-water certified reference materials in the range of 0.10 to 0.51% ABV was performed.

-- The repeatability ranged from 1.03 to 3.26% RSD

C. Data on robustness of enzyme response was presented.

-- The absorbance remains constant through 20 minutes after the reaction

D. Data on enzyme stability was presented.

--Enzymes producing the response are stable for several months in adequate storage conditions.