Kombucha ERP

AOAC Stakeholder Panel on Strategic Food Analytical Methods - Report on the Ethanol in Kombucha Expert Review Panel

Kombucha Method Reviews and Decisions

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Manuscript Title, Submitter and Reviewer

ERP Decisions

Consensus

KOM-04 Determination of Ethanol in Kombucha by Gas Chromatography-Flame Ionization Detector: Intra- Laboratory Validation

MOTION not to move this method to First Action OMA Status. (Mirzoian / Bhandari)

The ERP agreed not to move this method to First Action OMA Status for the following reasons: • Unclear how LOQ and LOD were evaluated/defined • Multiple definitions are lacking • Specificity and robustness were not addressed • No Method accuracy evaluation data using reference material available. • No data about analytical range, method precision. % Recovery data limited and require clarification as indicated in ERP review. • In summary, does not meet SMPR The ERP had no further recommendations for this method. The ERP agreed not to move this method to First Action Official Methods of Analysis Status. The ERP agreed that this method could be acceptable as a First Action OMA if additional precision data could be provided, specifically: • Repeatability study for minimum of eight (8) kombucha commercial samples. Range of 0.5% – 2% ABV. Minimum triplicate for two different days. • Provide data to demonstrate sample integrity with respect to ethanol concentration. The ERP agreed not to move this method to First Action Official Methods of Analysis Status. Key points: • Lacking safety precautions Lacking sufficient precision data •

7 in favor, 0 opposed, 0 abstentions.

The motion passed.

Submitted by: Xin Du, Murdoch University Primary Reviewer: Mirzoian Secondary Reviewer: Bhandari

KOM-05 Determination of Alcohol Content in Kombucha Tea by Headspace Solid Phase Micro-extraction and Gas Chromatography-Mass Spectrometry

MOTION to accept the KOM-05 First Action Requirements (Bhandari / Ebersole).

6 in favor, 0 opposed, 1 abstention.

The motion passed.

Submitted by: Katherine Stenerson, Millipore Sigma Primary Reviewer: Ebersole Secondary Reviewer: Jayabalan

Enzymic method for measurement of Ethanol in

MOTION not to move this method to First Action OMA Status. (Bhandari /

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