Low Lactose ERP - Review Book

AOAC SMPR ERPs - 2019 METHOD REVIEW FORM

Submission Date

2019-09-04 04:58:22

Name

Salvatore Parisi

E-mail

drparisi@inwind.it

Organization

Al Balqa Applied University

Title of Method

Quantitation of Six Common Food Sugars in Various Matrices by HPLC-MS

AOAC Candidate Method Number (e.g. ALN-01)

LAC-002

Applicable SMPR

Low Lactose AOAC SMPR 2018.009

I. Summary of the Method

This method has been proposed with the aim of determining six common mono and disaccharides (fructose, galactose, glucose, sucrose, maltose, and lactose) in various food, infant formula, feed and pet food matrices. The method can be evaluated with relation to AOAC SMPR 2018.009: Standard Method Performance Requirements (SMPRs®) for Lactose in Low-Lactose or Lactose Free Milk, Milk Products, and Products Containing Dairy Ingredients. It has to be noted that this approach concerns six carbohydrates including lactose. Consequently, another SMPR - AOAC SMPR® 2018.001 (Standard Method Performance Requirements (SMPRs®) for Sugars in Animal Feed, Pet Food, and Human Food) is applicable. In fact, Authors note that the method is also a re-submission for SUG-004. Basically, samples are extracted in 25% aqueous reagent alcohol at 60 °C under sonication and shaking. Carrez reagents are then introduced to precipitate protein. After centrifugation, the supernatant is diluted accordingly before LC-MS analysis. The method limit of quantitation (LOQ) is 0.005% (50 ppm) for each individual analyte. Yes, the applicability of the method supports the applicability of the SMPR. I would suggest the inclusion of “Low-Lactose or Lactose-Free” products in the “Purpose” field when speaking of “various food, infant formula, feed and pet food matrices”, with reference to AOAC SMPR 2018.009, section 2 (Applicability).

II. Review of the Method Only 1. Does the applicability of the method support the applicability of the SMPR? If not, please explain what is missing. 2. Does the analytical technique(s) used in the method meet the SMPR? If not, please specify how it differs from what is stated in the SMPR. 3. Are the definitions specified in the SMPR used and applied appropriately in the method? If no, please indicate how the terms are used.

Yes, the analytical technique used in the method meets the SMPR.

In general, the definitions specified in the SMPR are used and applied appropriately in the method. On the other hand, Limit of detection (LoD) and Repeatability (expressed as repeatability standard deviation or SDr) are not mentioned. Reference: AOAC SMPR 2018.009, section 4 (Definitions).