Low Lactose ERP - Review Book
AOAC SMPR ERPs - 2019 METHOD REVIEW FORM
Submission Date
2019-08-26 19:12:47
Name
Marina Torres
mtorres@latu.org.uy
Organization
Latu
Title of Method
Quantitation of Six Common Food Sugars in Various Matrices by HPLC-MS
AOAC Candidate Method Number (e.g. ALN-01)
LAC-002
Applicable SMPR
SMPR 2018.009
I. Summary of the Method
This method quantifies lactose, and five other sugars (fructose, galactose, glucose, sucrose and maltose) in various food, infant formula, feed and pet food matrices. Samples are extracted in 25% aqueous reagent alcohol at 60 °C under sonication and shaking. Carrez reagents are used to precipitate protein. After centrifugation, the dilutions are analyzed by LC-MS.
II. Review of the Method Only 1. Does the applicability of the method support the applicability of the SMPR? If not, please explain what is missing. 2. Does the analytical technique(s) used in the method meet the SMPR? If not, please specify how it differs from what is stated in the SMPR. 3. Are the definitions specified in the SMPR used and applied appropriately in the method? If no, please indicate how the terms are used. 4. Does the method, as written, contain all appropriate precautions and warnings related to the method's reagents, components, instrumentation, or method steps that may be hazardous? If no, please suggest wording or option(s).
Yes, the method could be used for matrices and levels mentioned in the SMPR.
The analytical technique of the method meets the SMPR.
Yes
Yes
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