Low Lactose ERP - Review Book

AOAC SMPR ERPs - 2019 METHOD REVIEW FORM

Submission Date

2019-08-25 03:58:28

Name

Lucien Duchateau

E-mail

lucien.duchateau@dsm.com

Organization

DSM Food Specialties

Title of Method

LactoSense

AOAC Candidate Method Number (e.g. ALN-01)

LAC-004

Applicable SMPR

AOAC SMPR 2018.009

I. Summary of the Method

The LactoSense method is a biosensor assay kit developed for the determination of lactose in a variety of lactose-free or low-lactose milk and dairy products produced with yeast neutral lactases. The LactoSense method was validated in single laboratory studies for calibration, interference, repeatability, recovery and robustness. Samples naturally containing lactose were evaluated in a method comparison study using an accredited high-performance anion- exchange chromatography with pulsed amperometric detection method as a reference method. On basis of the results obtained, the LactoSense method meets the requirements of SMPR 2018.009 and should be adopted as a First Action AOAC Official method

II. Review of the Method Only 1. Does the applicability of the method support the applicability of the SMPR? If not, please explain what is missing. 2. Does the analytical technique(s) used in the method meet the SMPR? If not, please specify how it differs from what is stated in the SMPR. 3. Are the definitions specified in the SMPR used and applied appropriately in the method? If no, please indicate how the terms are used.

Partly The LactoSense method is NOT applicable for 'lactose-free' infant formulas

Partly The lower limit of quantification (i.e. 5 mg/100 g) for lactose in infant formulas cannot be reached by the LactoSense method

yes