Micro December 2018

No confirmation is required, however, if necessary colonies may be isolated for further 1

identification. Lift the top film and pick the colony from the gel. Test using appropriate 2

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reference method procedures.

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Validation Study

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This validation study was conducted under the AOAC Research Institute Performance Tested 7

Method SM (PTM) program and the AOAC INTERNATIONAL Methods Committee Guidelines for 8

Validation of Microbiological Methods for Food and Environmental Surfaces (5). The 9

inclusivity/exclusivity study, robustness testing and matrix studies for all claimed matrixes were 10

conducted independently by Q Laboratories, Inc. (Cincinnati, OH). Product consistency and 11

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stability data will be submitted when available.

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Inclusivity/Exclusivity Testing

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The objective of this experiment was to determine the ability of the 3M Petrifilm Rapid E.

coli /Coliform Count Plate method to detect a variety of E. coli and non- E. coli coliform strains. A 17

total of 50 E. coli isolates and 50 non- E. coli coliform isolates were cultured in ISO Buffered 18

peptone water (BPW), prepared per ISO 6579 (6), at 37 ± 0.5°C for 24 ± 2 h. 19

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The purpose of exclusivity testing was to determine the ability of the 3M Petrifilm Rapid E.

coli /Coliform Count Plate method to discriminate target organisms from non-target organisms. 21

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