Micro December 2018

38 39 40 41 42 43 44 45 46 47 48 49 50

Escherichia coli Escherichia coli Escherichia coli Escherichia coli Escherichia coli Escherichia coli Escherichia coli Escherichia coli Escherichia coli Escherichia coli Escherichia coli Escherichia coli Escherichia coli

3M REC7 3M REC8 3M REC9 3M REC10 3M REC11 3M REC12 e 3M REC13 3M REC14 3M REC15 3M REC16 3M REC17 3M REC18 3M REC19

Food isolate Food isolate Food isolate Food isolate Food isolate Not available Not available Not available Not available Not available Not available Not available Not Available

55 19 25 38 17 31 41 51 77 47 30 39 21

61 29 23 35 24 40 40 52 87 53 49 37 27

53 17 19 26 21 62 54 58 93 48 61 44 38

58 22 21 35 20 27 38 47 75 48 32 41 19

62 26 20 34 25 37 40 50 88 49 52 40 23

58 18 18 30 22 64 55 49 90 46 57 48 31

a ATCC: American Type Culture Collection, Manassas, VA. b NRBC: NITE Biological Resource Center, Tokyo, Japan. c Origin not available. d QL: Q Laboratories Culture Collection, Cincinnati, OH. e 3M: 3M Food Safety, St. Paul, MN. d Atypical E. coli strain.

Table 2. 3M Petrifilm Rapid E. coli /Coliform Count Plate: E. coli inclusivity results vs. ISO 16649-2:2001

3M Petrifilm Rapid E. coli /Coliform Count Plate CFU/mL

ISO 16649-2:2001 CFU/mL

No.

Organism

Source

Origin Feces

30°C

37°C

42°C

30°C

37°C 44°C

1 2 3 4 5 6 7 8 9

Escherichia coli Escherichia coli Escherichia coli Escherichia coli Escherichia coli Escherichia coli Escherichia coli Escherichia coli Escherichia coli Escherichia coli Escherichia coli Escherichia coli Escherichia coli Escherichia coli Escherichia coli Escherichia coli Escherichia coli

ATCC a 8739 NBRC b 15034 ATCC 25922 ATCC 11229 ATCC 10536 ATCC 11303 ATCC 11775 QL d 11010-2 ATCC 14948 ATCC 23848 ATCC 33849 ATCC 35218 ATCC 35421 QL 41411.1 QL 41411.2 QL16154-1 QL 16154-2

64 38 49 38 56 82 89 49 92 99 60 93 79 66 73 56 7

60 51 34 37 84 82 91 42 98 97 25 55 90 63 73 82 69

46 49 49 28 69 94 95 39 99 99 15 59 93 30 78 79 51

60 37 48 39 54 80 84 53 90 99 10 62 93 77 64 72 58

63 49 34 38 78 87 92 41 95 99 28 57 94 61 75 79 73

43 51 47 32 68 95 94 40 99 98 16 60 91 31 75 80 49

Clinical specimen

Not available c

Feces

Not available Not available

Food, Minnesota Bottled water Not available Not available Not available Not available Bottled water Bottled water Canine, Tennessee Beef carcass sponge Raw ground beef

10 11 12 13 14 15 16 17

Page 32 of 61