Micro December 2018

Klebsiella pneumoniae

29 30 31 32 33 34 35 36 37 38 39 40 42 43 44 45 46 47 48 49 50

QL 11007-7 ATCC 33110 ATCC 19552 ATCC 55046 ATCC 27592 QL 11007-1 ATCC 14756 ATCC 13880 QL 16160-1 QL 16160-2 QL 16160-3 QL 16160-4 QL 16160-6 QL 16160-7 QL16160-8 QL 16160-9 QL 16160-10 QL 16160-11 QL 16160-12 QL 16160-13 QL 16160-14

Raw meat

92 13

98 19

82

94 16

96 20

83

Kluyvera intermedia

Surface water

2 0 1 3

4 0 3 6

Pantoea agglomerans g

Sewage

0 7

0

0 5

0

Rahnella aquatilis Serratia liquefaciens a Serratia marcescens a Serratia marcescens a Serratia marcescens a

Soil

13 97 89 97 54 65 95 91 43 86 78 38 75 92 68 32 13 10

11 95 87 96 55 63 94 90 46 89 76 40 81 93 65 30 16

Milk

86 54 89 32 97 89 89 31 62 40 76 31 55 33 46 13 69

83 51 85 29 91 88 86 34 65 41 72 33 56 37 48 15 70

Bottled water Human isolate Human isolate Mixed frozen vegetables Frozen greens Chicken nuggets

76 70

79 69

5

4

Escherichia coli h Escherichia coli Escherichia coli Escherichia coli Escherichia coli Escherichia coli Escherichia coli Escherichia coli Escherichia coli Escherichia coli Escherichia coli Escherichia coli Escherichia coli

80 94 73 72 50 48 48 40 77 47

78 93 76 73 54 50 46 39 76 47

Beef carcass sponge Flavored seasoning Chicken carcass rinsate

Fresh baby spinach

Raw pork

Raw beef trimmings Raw beef trimmings Irrigation rinsate water Beef carcass sponge Chicken carcass rinsate

3

2

46 30

45 31

8

a Typical red coliform colonies produced but without gas production b ATCC: American Type Culture Collection, Manassas, VA. c Origin not available.

d QL: Q Laboratories Culture Collection, Cincinnati, OH. e NBRC: NITE Biological Resource Center, Tokyo, Japan. f NCTC: National Collection Type Cultures, Porton Down, Salisbury, UK. g Isolate not detected on 3M™ Petrifilm Rapid E. coli /Coliform Count Plate and VRB. h Typical blue, blue-green colonies produced for all E. coli strains tested.

Table 7. 3M Petrifilm Rapid E. coli /Coliform Count Plate: Coliform exclusivity results vs. BAM Chapter 4

3M Petrifilm Rapid E. coli /Coliform Count Plate CFU/mL

BAM Ch. 4 CFU/mL 32°C 35°C 42°C

No.

Organism

Source

Origin Urine

32°C

35°C

42°C

Acinetobacter baumanii

1 2 3 4 5

ATCC a 19606 QL b 17041-8 ATCC 8750 ATCC 6464 ATCC 6633

<1 <1 <1 <1 <1

<1 <1 <1 <1 <1

<1 <1 <1 <1 <1

<1 <1 <1 <1 <1

<1 <1 <1 <1 <1

<1 <1 <1 <1 <1

Aeromonas viridans Alcaligenes faecalis

Raw milk isolate Not available c

Bacillus cereus Bacillus subtilis

Soil

Not available

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