Micro December 2018

SUMMARY OF COMMENTS  OMAMAN‐46: Enumeration of E. coli and Coliform

ER 5 It is an economic method that does not need specific instruments for its performing, so it is very  accessible to laboratories of low complexity ER 6 Manuscript is of normal quality ER 7 Generally well written and clearly presented. ER 8 Well written. ER 9 Well written and thorough Cons/Weaknesses of the Manuscript: ER 1 N/A ER 2 On page two of the collaborative manuscript, line 2.  Is it necessary to individually list the  incubation temperatures?  Practically, the plates can be incubated at 30‐37C +/‐ 1C. ER 3 Wondering if a highly contaminated product of some pathogens lines 1‐3 as described on page 7  may cause any issues. ER 4 none ER 5 No ER 6 NA ER 7 The title implies that an E. coli count and a total coliform count can be obtained. This does not  seem to be exactly so. A total coliform count can be obtained (blue plus red+/‐gas colonies).  However, the blue+/‐gas only count will miss glucuronidase negative E. coli O157 but will include  atypical non‐E. coli coliforms (glucuronidase positives). Suggest changing title to "Evaluation of 3M  Petrifilm Rapid E.coli/Coliform Count Plate for enumeration of total coliforms and enumeration of  glucuronidase positive E. coli plus atypical non‐E. coli coliforms: Collaborative Study." Also could  pick 10 blue colonies and check if any are atypical non‐E. coli coliforms.

ER 8 ER 9 ER 1 ER 2 ER 3 ER 4 ER 5 ER 6 ER 7 ER 8 ER 9

References not correct.

Nonr Is the Validation Study Manuscript in a format acceptable to AOAC?

Yes Yes Yes Yes Yes Yes Yes Yes

Yes Is the method described in sufficient detail so that it is relatively easy to understand, including  equations and procedures for calculation of results (are all terms explained)? ER 1 Yes ER 2 Yes ER 3 Yes ER 4 Yes

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