Micro December 2018

It is recommended that the 3M Petrifilm Rapid E. coli /Coliform Count Plate be adopted as 1 Official First Action status for the enumeration of E. coli and coliforms in a broad range of foods 2 and environmental surface established from testing pasteurized whole milk, butter, non-fat dry 3 milk, raw ground pork, lamb chop, raw ground chicken, chicken carcass rinsate, shell eggs, 4 liquid egg whites, powdered egg whites, sprouts, cranberries, infant formula with probiotics, 5 infant formula without probiotics, infant rice cereal without probiotics, dry dog kibble, dry cat 6 food, flour, chocolate chip cookie dough, raw ground beef (73% lean), raw frozen chicken 7 wings, raw milk, whole liquid egg, tuna sushi, smoked salmon, bunched spinach, pasteurized 8 carrot juice, ready-made sandwiches, raw vegetable salad with dressing, chicken feed, soybean 9 meal, stainless steel environmental sponges and sealed concrete environmental sponges. 12 13 We would like to extend a sincere thank you to the following collaborators for their dedicated 14 participation in this study: 17 Virendra Gohil & Krishnarl Suntharesan – Maxxam Analytics – Mississauga, Ontario Canada 18 Nallely Hernandez Alvarez - Cencon Centro de Control- Guadalajara, Mexico. 19 Alexandra Calle – Texas Tech University, Dept. of Animal Food Sciences – Lubbock, TX 20 Sara Diederich & Steve Kuchenberg – Cherney Microbiological Services – Green Bay, WI 21 Satoshi Ishii – University of Minnesota, Gortner Lab of Biochemistry – Saint Paul, MN 22 Adam Donkers, Robbie J. Smith & Diane S. Wood – Maple Leaf Foods – Puslinch, Ontario, 23 Canada 24 Sabrina Corti & Mirjam Hochreuter – University of Zurich, Zurich Switzerland 10 11 Acknowledgements 15 16 Megan Brown - Covance Laboratories, Madison, WI Washington State Department of Health. Questions & Answers: Coliform Bacteria and Drinking Water. https://www.doh.wa.gov/Portals/1/Documents/Pubs/331-181.pdf 31 32 (2) Official Methods of Analysis of AOAC INTERNATIONAL. (2016) 20th Ed., Appendix 33 J: AOAC INTERNATIONAL Methods Committee Guidelines for Validation of 34 Microbiological Methods for Food and Environmental Surfaces, AOAC 35 INTERNATIONAL, Gaithersburg, MD, 36 http://www.eoma.aoac.org/app_j.pdf (Accessed August 2018) 37 38 (3) Feng, P., Weagant, S.D., Grant, M.A, Burkhardt, W. (2002) in Bacteriological Analytical 39 Manual ,8th Ed., Robert I. Merker. Center for Food Safety and Applied Nutrition. College 40 Park, MD. 41 https://www.fda.gov/Food/FoodScienceResearch/LaboratoryMethods/ucm064948.htm 42 (Accessed August 2018) 43 44 (4) International Organization for Standardization. (2006). - Microbiology of food and animal 45 feeding stuffs – Horizontal method for the enumeration of coliforms—Colony-count 46 technique. Geneva, Switzerland, ISO 4832:2006. www.iso.org (Accessed August 2018) 47 (Accessed August 2018) 25 26 27 28 29 30 References

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